采用带有多重激光诱导荧光检测的多通道微芯片的毛细管电泳进行并行分离。
Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser-induced fluorescence detection.
机构信息
Department of Chemistry, University of Cincinnati, Cincinnati, OH, USA.
出版信息
Electrophoresis. 2010 Aug;31(16):2796-803. doi: 10.1002/elps.201000030.
Abstract
Parallel separations using CE on a multilane microchip with multiplexed LIF detection is demonstrated. The detection system was developed to simultaneously record data on all channels using an expanded laser beam for excitation, a camera lens to capture emission, and a CCD camera for detection. The detection system enables monitoring of each channel continuously and distinguishing individual lanes without significant crosstalk between adjacent lanes. Multiple analytes can be determined in parallel lanes within a single microchip in a single run, leading to increased sample throughput. The pK(a) determination of small molecule analytes is demonstrated with the multilane microchip.
摘要
采用带有多重 LIF 检测的多通道微芯片进行 CE 平行分离得到了验证。该检测系统是为了同时使用扩展的激光束进行激发、相机镜头捕获发射光以及 CCD 相机进行检测，从而对所有通道的数据进行记录而开发的。该检测系统能够对每个通道进行连续监测，并区分各个通道，而相邻通道之间的串扰则可以忽略不计。在单个微芯片中可以在单个运行中并行通道中同时确定多种分析物，从而提高了样品通量。本文还使用多通道微芯片验证了小分子分析物的 pK(a)值的测定。
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