Aladzhov E, Logofetov A
Eksp Med Morfol. 1990;29(2):8-12.
The change of ceruloplasmin level of blood serum of guinea-pigs was studied on the 12th h after irradiation with gamma-rays from 60Co, using doses of 1-10 Gy, power of the dose of 0.68 Gy/min. The enzyme level was raised by the increase of the irradiation dose as it was reduced by the dose of 10 Gy in comparison with the control animals. This was probably due to inactivation of the molecules of ceruloplasmin by radicals and peroxides, obtained by this hyperlethal dose. Sera proteins showed a change in the increase of the dose: a reduction of albumins and an increase in beta- and gamma-globulins. Alpha-2-globulins, with which ceruloplasmin moved electrophoretically at low doses, showed slowly a slow increase in comparison with the control value, but at high doses-phase globulins. Oxidase activity of ceruloplasmin was determined by 01 todianisidine, but sera proteins-by agarose electrophoresis. The level of s ceruloplasmin in animals, irradiated by LD50 = 4.1 Gy, showed chronological change-an increase at early periods, maximum on the 12th h and a reduction at late periods close to the normal range. The level of serum copper followed the change in the enzyme level by analogy.
用剂量为1 - 10 Gy、剂量率为0.68 Gy/min的60Coγ射线照射豚鼠，在照射后12小时研究其血清铜蓝蛋白水平的变化。与对照动物相比，随着照射剂量增加，该酶水平升高，但在10 Gy剂量时降低。这可能是由于该超致死剂量产生的自由基和过氧化物使铜蓝蛋白分子失活所致。血清蛋白显示出随剂量增加的变化：白蛋白减少，β - 和γ - 球蛋白增加。低剂量时与铜蓝蛋白一起电泳的α2 - 球蛋白与对照值相比缓慢增加，但在高剂量时呈阶段性变化。铜蓝蛋白的氧化酶活性用邻联茴香胺测定，血清蛋白用琼脂糖电泳测定。受LD50 = 4.1 Gy照射的动物体内铜蓝蛋白水平呈现出随时间的变化——早期升高，第12小时达到最高，后期降低至接近正常范围。血清铜水平与酶水平的变化类似。
