Aladzhov E
Vet Med Nauki. 1987;24(6):71-4.
Studied were the changes in the concentration of ceruloplasmin through altering its oxidase activity in the blood serum by gamma treatment, using a 60Co source at the rate of 3 Gy and dose power of 0.68 Gy/min-1. The oxidase activity of the enzyme was determined colorimetrically with an o-dianisidine substrate. The concentration of ceruloplasmin rose from 34 E/l prior to irradiation to 129 E/l following treatment. The analyses were carried out immediately before using gamma rays and at the 30th min, at the 15th and 40th hour, and on the 10th, 17th, and 30th day after that. By the 30th day the ceruloplasmin level was found to drop up to 90 E/l. The serum proteins also changed their values following treatment. Albumin and gamma-globulins dropped from 52.3 to 43.2 per cent and from 20.1 to 13.1 per cent, respectively. Alfa-1 rose from 8.5 to 9.8 per cent, alfa-2 globulins--from 13.0 to 17.7 per cent, and beta-globulins--from 6.0 to 15.1 per cent. Alfa-2 globulins were shown to follow the same pattern of rising as ceruloplasmin and serum copper. As a result of the irradiation disease that developed after treatment a paraclinical picture of an acute inflammatory process set in.
研究了通过使用60Co源以3 Gy的剂量率和0.68 Gy/min-1的剂量功率进行γ处理来改变血清中铜蓝蛋白的氧化酶活性，从而观察铜蓝蛋白浓度的变化。用邻联茴香胺底物通过比色法测定该酶的氧化酶活性。铜蓝蛋白浓度从照射前的34 E/l升至处理后的129 E/l。在使用γ射线之前、处理后第30分钟、第15和40小时以及之后的第10、17和30天立即进行分析。到第30天，发现铜蓝蛋白水平降至90 E/l。处理后血清蛋白的值也发生了变化。白蛋白和γ球蛋白分别从52.3%降至43.2%和从20.1%降至13.1%。α1从8.5%升至9.8%，α2球蛋白从13.0%升至17.7%，β球蛋白从6.0%升至15.1%。结果显示α2球蛋白与铜蓝蛋白和血清铜呈现相同的上升模式。处理后由于辐射引发的疾病，出现了急性炎症过程的临床旁证表现。
