[Enzymatic activity of ceruloplasmin in gamma-irradiated lambs].

Studied were the changes in the concentration of ceruloplasmin through altering its oxidase activity in the blood serum by gamma treatment, using a 60Co source at the rate of 3 Gy and dose power of 0.68 Gy/min-1. The oxidase activity of the enzyme was determined colorimetrically with an o-dianisidine substrate. The concentration of ceruloplasmin rose from 34 E/l prior to irradiation to 129 E/l following treatment. The analyses were carried out immediately before using gamma rays and at the 30th min, at the 15th and 40th hour, and on the 10th, 17th, and 30th day after that. By the 30th day the ceruloplasmin level was found to drop up to 90 E/l. The serum proteins also changed their values following treatment. Albumin and gamma-globulins dropped from 52.3 to 43.2 per cent and from 20.1 to 13.1 per cent, respectively. Alfa-1 rose from 8.5 to 9.8 per cent, alfa-2 globulins--from 13.0 to 17.7 per cent, and beta-globulins--from 6.0 to 15.1 per cent. Alfa-2 globulins were shown to follow the same pattern of rising as ceruloplasmin and serum copper. As a result of the irradiation disease that developed after treatment a paraclinical picture of an acute inflammatory process set in.