Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Phytomedicine. 2011 Jan 15;18(2-3):110-8. doi: 10.1016/j.phymed.2010.07.001. Epub 2010 Aug 23.
In the present study, the antiviral activity of pinostrobin towards herpes simplex virus-1 (HSV-1) was investigated by MTT assay and atomic force microscopy. Pinostrobin can inhibit HSV-1 replication with 50% effective concentration (EC(50)) of 22.71 ± 1.72 μg/ml. MTT assay showed HSV-1 was significantly inhibited when pretreated with pinostrobin, with the inhibition of 85.69 ± 2.59%. Significant changes in morphology and size of HSV-1 were observed by atomic force microscopy (AFM) in response to pinostrobin treatment. AFM topography and phase images showed that with increasing time, the envelope was shedded and damaged, finally leading to virus inactivation. With increasing concentration, pinostrobin caused a gradual leakage, also contributing to breakage of the envelope and virus inactivation. Treatment effect of oral pinostrobin in vivo showed that pinostrobin (50mg/kg/dose) possesses definite therapeutical effect in the development of lesion score. In general, the results showed that AFM represents a powerful technique for the investigation of morphology and size of HSV-1 treated by antiviral agents. AFM is applicable to study chemically induced morphological changes at the nanometer level.
在本研究中，通过 MTT 检测法和原子力显微镜（AFM）研究了紫檀芪对单纯疱疹病毒-1（HSV-1）的抗病毒活性。紫檀芪对 HSV-1 的复制具有抑制作用，其 50%有效浓度（EC（50））为 22.71 ± 1.72μg/ml。MTT 检测法表明，紫檀芪预处理可显著抑制 HSV-1，抑制率为 85.69 ± 2.59%。AFM 观察到 HSV-1 的形态和大小在紫檀芪作用下发生了明显变化。AFM 形貌和相图显示，随着时间的增加，包膜逐渐脱落和受损，最终导致病毒失活。随着浓度的增加，紫檀芪导致逐渐渗漏，也有助于包膜破裂和病毒失活。体内口服紫檀芪的治疗效果表明，紫檀芪（50mg/kg/剂量）在病变评分的发展中具有一定的治疗作用。总的来说，结果表明 AFM 代表了一种用于研究抗病毒药物处理后的 HSV-1 形态和大小的强大技术。AFM 适用于研究化学诱导的纳米级形态变化。
