Greninger Alexander L, Holtz Lori, Kang Gagandeep, Ganem Donald, Wang David, DeRisi Joseph L
Departments of Molecular Microbiology and Pathology and Immunology, Howard Hughes Medical Institute,Washington University, St. Louis, MO 63110, USA.
Clin Vaccine Immunol. 2010 Oct;17(10):1584-8. doi: 10.1128/CVI.00152-10. Epub 2010 Aug 25.
Klassevirus is a proposed new genus of picornavirus that has been associated with pediatric diarrhea. In this study, we used recombinant klassevirus 3C protease as the capture antigen for an indirect serological enzyme-linked immunosorbent assay (ELISA). Four of six klassevirus reverse transcription (RT)-PCR-positive individuals demonstrated seroconversion against the 3C protease, suggesting that klassevirus infection and replication occur in humans. Additional screening of 353 samples from an age-banded serological cohort from two St. Louis hospitals indicated a seroprevalence of 6.8%.
克拉斯病毒是一种拟议中的微小核糖核酸病毒新属，与小儿腹泻有关。在本研究中，我们使用重组克拉斯病毒3C蛋白酶作为捕获抗原，用于间接血清学酶联免疫吸附测定（ELISA）。6名克拉斯病毒逆转录（RT）-PCR阳性个体中有4人显示出针对3C蛋白酶的血清转化，这表明克拉斯病毒在人类中发生感染和复制。对来自圣路易斯两家医院按年龄分组的血清学队列中的353份样本进行的额外筛查显示血清阳性率为6.8%。
