Yu Jie-Mei, Ao Yuan-Yun, Liu Na, Li Li-Li, Duan Zhao-Jun
Institute for Viral Diseases Control and Prevention, China CDC, Beijing, China.
PLoS One. 2015 Jul 20;10(7):e0130977. doi: 10.1371/journal.pone.0130977. eCollection 2015.
Salivirus was recently discovered in children with gastroenteritis and in sewage. Though a causative role for salivirus in childhood gastroenteritis was suggested in the previous study, the relationship between salivirus and acute gastroenteritis has not yet been clearly clarified. The sewage strain reported by Ng, although represented by incomplete genome sequencing data, was distinct from previously reported saliviruses, and had not previously been detected in humans. A case-control study examining 461 paired stool samples from children with diarrhea and healthy controls (1:1) was conducted in this study. Also, common diarrheal viruses were detected and complete genome of a salivirus was determined. Results showed that salivirus was detected in 16 (3.5%) and 13 (2.8%) of the case and control samples, respectively; no differences in detection rates (p=0.571) or mean values of viral loads (p=0.400) were observed between the groups. Multivariate Cox regression revealed no association between salivirus and gastroenteritis (p=0.774). The data also demonstrated that salivirus infection did not exacerbate clinical symptoms of gastroenteritis in children. Furthermore, complete genome sequence of a salivirus recovered from the feces of a child with diarrhea (i.e., SaliV-FHB) shared a 99% nucleotide identity with the sewage strain. In conclusion, a paired case-control study did not support a causative role for salivirus strains detected in this study with pediatric gastroenteritis. This study also demonstrated that all known saliviruses can be detected in the feces of children with or without gastroenteritis.
唾液病毒最近在患有肠胃炎的儿童和污水中被发现。尽管先前的研究表明唾液病毒在儿童肠胃炎中可能起致病作用，但唾液病毒与急性肠胃炎之间的关系尚未完全阐明。Ng报道的污水菌株，虽然其基因组测序数据不完整，但与先前报道的唾液病毒不同，且此前未在人类中检测到。本研究开展了一项病例对照研究，检测了461对腹泻儿童和健康对照（1:1）的粪便样本。此外，还检测了常见的腹泻病毒，并测定了一种唾液病毒的完整基因组。结果显示，病例组和对照组样本中分别有16例（3.5%）和13例（2.8%）检测到唾液病毒；两组之间在检出率（p=0.571）或病毒载量平均值（p=0.400）上未观察到差异。多变量Cox回归显示唾液病毒与肠胃炎之间无关联（p=0.774）。数据还表明，唾液病毒感染并未加重儿童肠胃炎的临床症状。此外，从一名腹泻儿童粪便中分离出的唾液病毒（即SaliV-FHB）的完整基因组序列与污水菌株的核苷酸同一性为99%。总之，一项配对病例对照研究不支持本研究中检测到的唾液病毒株与小儿肠胃炎之间存在致病关系。本研究还表明，在患有或未患有肠胃炎的儿童粪便中均可检测到所有已知的唾液病毒。
