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比较肺炎链球菌与草绿色链球菌的转录组分析。

Comparative transcriptomic analysis of streptococcus pseudopneumoniae with viridans group streptococci.

机构信息

Department of Microbiology & Research Center for Medical Sciences, Chung-Ang University College of Medicine, Seoul, 156-756, Republic of Korea.

出版信息

BMC Microbiol. 2012 Jul 6;12:77. doi: 10.1186/1471-2180-12-77.

DOI:10.1186/1471-2180-12-77
PMID:22607240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3391171/
Abstract

BACKGROUND

Streptococcus pseudopneumoniae, is a novel member of the genus Streptococcus, falling close to related members like S. pneumoniae, S. mitis, and S. oralis. Its recent appearance has shed light on streptococcal infections, which has been unclear till recently. In this study, the transcriptome of S. pseudopneumoniae CCUG 49455T was analyzed using the S. pneumoniae R6 microarray platform and compared with those of S. pneumoniae KCTC 5080T, S. mitis KCTC 3556T, and S. oralis KCTC 13048T strains.

RESULTS

Comparative transcriptome analysis revealed the extent of genetic relatedness among the species, and implies that S. pseudopneumoniae is the most closely related to S. pneumoniae. A total of 489, 444 and 470 genes were upregulated while 347, 484 and 443 were downregulated relative to S. pneumoniae in S. pseudopneumoniae, S. oralis and S. mitis respectively. Important findings were the up-regulation of TCS (two component systems) and transposase which were found to be specific to S. pseudopneumoniae.

CONCLUSIONS

This study provides insight to the current understanding of the genomic content of S. pseudopneumoniae. The comparative transcriptome analysis showed hierarchical clustering of expression data of S. pseudopneumoniae with S. pneumoniae and S. mitis with S. oralis. This proves that transcriptional profiling can facilitate in elucidating the genetic distance between closely related strains.

摘要

背景

肺炎链球菌是链球菌属的一个新成员，与肺炎链球菌、缓症链球菌和口腔链球菌等相关成员关系密切。其最近的出现揭示了直到最近才清楚的链球菌感染。在这项研究中，使用肺炎链球菌 R6 微阵列平台分析了 S. pseudopneumoniae CCUG 49455T 的转录组，并与肺炎链球菌 KCTC 5080T、缓症链球菌 KCTC 3556T 和口腔链球菌 KCTC 13048T 菌株的转录组进行了比较。

结果

比较转录组分析揭示了这些物种之间遗传关系的程度，并暗示 S. pseudopneumoniae 与肺炎链球菌最为密切相关。与肺炎链球菌相比，S. pseudopneumoniae、口腔链球菌和缓症链球菌中分别有 489、444 和 470 个基因上调，347、484 和 443 个基因下调。重要的发现是 TCS（双组分系统）和转座酶的上调，这两种基因被发现是 S. pseudopneumoniae 所特有的。

结论

这项研究提供了对 S. pseudopneumoniae 基因组内容的最新理解。比较转录组分析显示，S. pseudopneumoniae 的表达数据与肺炎链球菌呈层次聚类，S. mitis 的表达数据与口腔链球菌呈层次聚类。这证明转录谱分析有助于阐明亲缘关系密切的菌株之间的遗传距离。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d33/3391171/f48b7efd777f/1471-2180-12-77-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d33/3391171/0bcae44a0f32/1471-2180-12-77-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d33/3391171/70954b67424f/1471-2180-12-77-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d33/3391171/ff5e779ef9f5/1471-2180-12-77-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d33/3391171/f48b7efd777f/1471-2180-12-77-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d33/3391171/0bcae44a0f32/1471-2180-12-77-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d33/3391171/70954b67424f/1471-2180-12-77-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d33/3391171/ff5e779ef9f5/1471-2180-12-77-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d33/3391171/f48b7efd777f/1471-2180-12-77-4.jpg

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