Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
Int J Hematol. 2010 Sep;92(2):326-33. doi: 10.1007/s12185-010-0668-8. Epub 2010 Aug 26.
Multidrug resistance (MDR) in leukemia is commonly associated with the expression of a transmembrane protein, P-glycoprotein (P-gp). In this study, two monoclonal antibodies (mAbs) specific for the extracellular domain of P-gp were generated. By employing the generated mAbs, a two-color lysed whole blood flow cytometric method for surface P-gp and an efficient sandwich ELISA for soluble P-gp determinations were established. By using the established methods, surface and soluble P-gp were detected in several leukemia patients. The presence of soluble P-gp could be used to identify the P-gp surface expression patients. Detection of soluble P-gp reported provides a new basis that may lead to a better understanding of the MDR mechanism in leukemia.
白血病中的多药耐药（MDR）通常与跨膜蛋白 P-糖蛋白（P-gp）的表达有关。在这项研究中，生成了两种针对 P-gp 细胞外结构域的单克隆抗体（mAbs）。通过使用生成的 mAbs，建立了用于表面 P-gp 的双色裂解全血流式细胞术方法和用于可溶性 P-gp 测定的高效夹心 ELISA。使用建立的方法，在几个白血病患者中检测到了表面和可溶性 P-gp。可溶性 P-gp 的存在可用于鉴定 P-gp 表面表达的患者。可溶性 P-gp 的检测报告提供了一个新的基础，可能有助于更好地理解白血病中的 MDR 机制。
