Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
J Biomed Mater Res A. 2010 Oct;95(1):33-9. doi: 10.1002/jbm.a.32763.
Our previous study demonstrated that calcium-bonded titanium surface (Ca-Ti) can be obtained by hydrothermal reaction between titanium (Ti) and CaCl(2) and that bone-apatite like formation was observed after immersion in simulated body fluid. The purpose of the study was to determine the in vivo response to Ca-Ti surface using a rodent tibia model. Cylinders of commercially pure Ti were divided into three groups: (1) untreated group; (2) NaOH+hTi group: soaked in 5 mol/L NaOH solution at 60 degrees C then heated at 400 degrees C for 1 h; and (3) Ca-Ti group: hydrothermally treated in the presence of 10 mmol/L CaCl(2) at 200 degrees C for 24 h. The cylinders implanted in surgically created defects in tibias of 8-week old male Wistar rats were retrieved after 1, 2, and 4 weeks. Histomorphometric evaluations were made on stained decalcified thin sections. Results showed that at 1, 2, and 4 week after implantation, respectively, bone contact was 55.2 +/- 16.4%, 88.1 +/- 9.9%, and 96.1 +/- 4.8% for Ca-Ti implants, 5.7 +/- 5.3%, 19.9 +/- 1.2%, 57.4 +/- 4.8% for untreated; and 27.2 +/- 0.7%, 70.9 +/- 7.7%, and 96.0 +/- 5.1% for NaOH+hTi implants. These results suggest that hydrothermal treatment with CaCl(2) provides a bioactive Ca-Ti bonded surface that allows bone formation greater than that obtained with NaOH+heat treated Ti surfaces.
我们之前的研究表明，钛（Ti）和 CaCl2 之间的水热反应可以生成钙结合的钛表面（Ca-Ti），并且在浸入模拟体液后观察到类似骨磷灰石的形成。本研究的目的是使用啮齿动物胫骨模型确定 Ca-Ti 表面的体内反应。商业纯 Ti 圆柱体分为三组：（1）未处理组；（2）NaOH+hTi 组：在 5mol/L NaOH 溶液中于 60°C 下浸泡，然后在 400°C 下加热 1 小时；和（3）Ca-Ti 组：在 200°C 下于 10mmol/L CaCl2 存在下水热处理 24 小时。将圆柱体植入 8 周龄雄性 Wistar 大鼠手术创建的胫骨缺陷中，分别在植入后 1、2 和 4 周取出。对染色脱钙的薄片进行组织形态计量评估。结果表明，植入后 1、2 和 4 周，Ca-Ti 植入物的骨接触率分别为 55.2±16.4%、88.1±9.9%和 96.1±4.8%，未处理组分别为 5.7±5.3%、19.9±1.2%和 57.4±4.8%，NaOH+hTi 植入物分别为 27.2±0.7%、70.9±7.7%和 96.0±5.1%。这些结果表明，CaCl2 的水热处理提供了一种具有生物活性的 Ca-Ti 结合表面，可促进骨形成，超过了 NaOH+热处理 Ti 表面获得的骨形成。
