Ogawa T, Ohara K, Shimizu H
Department of Ophthalmology, Jichi Medical School, Tochigi, Japan.
Nippon Ganka Gakkai Zasshi. 1990 Nov;94(11):1001-6.
We studied the correlation between photon counts of the aqueous flare and total aqueous protein concentrations in rabbit eyes. After mydriasis, a predetermined volume of the aqueous humor was aspirated and photon counts of the secondary aqueous was measured using a laser flare-cell meter. In vitro measurements of total protein concentrations showed that step-wise changes in withdrawal volume ranging from 50 to 200 microliters induced corresponding rises of the total protein concentrations in the secondary aqueous, with the mean total protein concentrations ranging from 220 to 1980 mg/dl. A ratio of albumin to globulin concentrations (A/G) ranged from 1.0 to 3.6, but they did not show any significant correlation with the aqueous total protein concentrations. The mean photon counts in the secondary aqueous ranged from 40 to 343/msec. A statistical analysis showed that photon counts correlated well with the total protein concentrations. The linear regression equation was X = Y1.07 x 3.33 where X and Y indicate protein concentrations and photon counts, respectively (r = 0.92, p less than 0.001). The regression equation might be used to estimate the total aqueous protein concentrations in laser flare-cell measurements in vivo. The variation of A/G ratio affected the photon count values, and the total protein concentration might be within 3/2 and 1/2 of the estimated value.
我们研究了兔眼房水闪光的光子计数与房水总蛋白浓度之间的相关性。散瞳后，抽取预定体积的房水，并用激光房水细胞仪测量二次房水的光子计数。体外总蛋白浓度测量显示，抽取体积从50微升至200微升的逐步变化导致二次房水中总蛋白浓度相应升高，平均总蛋白浓度范围为220至1980mg/dl。白蛋白与球蛋白浓度之比（A/G）范围为1.0至3.6，但它们与房水总蛋白浓度未显示出任何显著相关性。二次房水中的平均光子计数范围为40至343/毫秒。统计分析表明，光子计数与总蛋白浓度密切相关。线性回归方程为X = Y1.07×3.33，其中X和Y分别表示蛋白浓度和光子计数（r = 0.92，p<0.001）。该回归方程可用于估计体内激光房水细胞测量中的房水总蛋白浓度。A/G比值的变化影响光子计数值，总蛋白浓度可能在估计值的3/2和1/2范围内。
