Saari K M, Guillén-Monterrubio O M, Hartikainen J, Hämäläinen M M, Taskinen K
Department of Ophthalmology, University of Turku, Finland.
Acta Ophthalmol Scand. 1997 Feb;75(1):63-6. doi: 10.1111/j.1600-0420.1997.tb00252.x.
We studied the correlation between laser flare cell meter photon count/ms and actual protein concentration both in vitro and in vivo. Laser flare cell meter measurement of human albumin concentrations of 0 to 10 g/l showed photon counts/ms from 0.3 +/- 0.3 to 78.9 +/- 3.9. There was a statistically highly significant linear correlation between photon count/ms and human albumin concentration (r = 0.98, p = 0.0001). Laser flare cell meter measurements were done on 39 cataract patients with the mean age of 77.9 +/- 6.7 years. Aqueous humour obtained by peroperative paracentesis was analysed for total protein. The mean photon count/ms before pupillary dilatation was 11.93 +/- 6.03. There was a significant linear correlation (r = 0.4, p = 0.019) between the photon count/ms after pupillary dilatation (mean 14.73 +/- 12.9, range 2.6-62.4) and anterior chamber protein concentration (mean 0.62 +/- 0.27, range 0.23-1.3 g/l) with the linear regression formula being y = 0.231 x -1.105 where y = log protein concentration (g/l) and x = log of photon count/ms. Laser flare cell meter photon counts/ms may be converted into an equivalent anterior chamber total protein concentration using this formula.
我们在体外和体内研究了激光闪辉细胞仪光子计数/毫秒与实际蛋白质浓度之间的相关性。激光闪辉细胞仪对人白蛋白浓度在0至10 g/l范围内的测量显示，光子计数/毫秒从0.3±0.3至78.9±3.9。光子计数/毫秒与人白蛋白浓度之间存在统计学上高度显著的线性相关性（r = 0.98，p = 0.0001）。对39例平均年龄为77.9±6.7岁的白内障患者进行了激光闪辉细胞仪测量。对手术中通过前房穿刺获得的房水进行总蛋白分析。瞳孔散大前的平均光子计数/毫秒为11.93±6.03。瞳孔散大后的光子计数/毫秒（平均14.73±12.9，范围2.6 - 62.4）与前房蛋白质浓度（平均0.62±0.27，范围0.23 - 1.3 g/l）之间存在显著的线性相关性（r = 0.4，p = 0.019），线性回归公式为y = 0.231x - 1.105，其中y = log蛋白质浓度（g/l），x = log光子计数/毫秒。使用该公式，激光闪辉细胞仪的光子计数/毫秒可转换为等效的前房总蛋白浓度。
