Yoshitomi T, Wong A S, Daher E, Sears M L
Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, CT 06510.
Jpn J Ophthalmol. 1990;34(1):57-62.
The laser flare-cell meter is a newly developed instrument designed to measure anterior chamber protein concentration in vivo. There was a linear relationship between photon count measured with this instrument and the concentration of bovine serum albumin. Measurements were taken in 29 eyes of normal subjects divided into two groups. Group 1 (15 eyes) had brown or dark irises and Group 2 (14 eyes) had blue or green irises. Photon counts obtained from Groups 1 and 2 were not significantly different. Aqueous protein concentrations estimated from standard curves were 15.7 and 18.6 mg/100 ml for Groups 1 and 2, respectively. On the other hand, the background value obtained from Group 2 was about twice the value from Group 1. These results indicate that background scattering has little effect on photon count. There was a good correlation between photon counts measured with the laser flare-cell meter and the evaluation of flare made by conventional microscopic observations. It was concluded that this instrument will be useful for clinical research studies.
激光闪辉细胞仪是一种新开发的用于在体内测量前房蛋白浓度的仪器。用该仪器测得的光子计数与牛血清白蛋白浓度之间存在线性关系。对29只正常受试者的眼睛进行了测量，并将其分为两组。第1组（15只眼）虹膜为棕色或深色，第2组（14只眼）虹膜为蓝色或绿色。第1组和第2组获得的光子计数无显著差异。根据标准曲线估计，第1组和第2组的房水蛋白浓度分别为15.7和18.6mg/100ml。另一方面，第2组获得的背景值约为第1组的两倍。这些结果表明背景散射对光子计数影响很小。用激光闪辉细胞仪测得的光子计数与传统显微镜观察对闪辉的评估之间存在良好的相关性。得出结论，该仪器将有助于临床研究。
