Luo D
Research Laboratory of Otology, Shanghai Second Medical University.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1990 Oct;25(5):281-2, 318.
Using the ultracytochemical technique, we found the lysosome with high electronic density just beneath the cuticle plate in all normal hair cells; but in ototoxic group, the lysosome became multivesicular, expanded and displaced in hair cells. Occasionally the vesicles of the lysosome fused. In some of the hair cells, the cytoplasm dissolved, the membrane ruptured, the contents of the cells leaked out. In these cells the lysosome disappeared. We assume that the vesicle in the lysosome may be kanamycin and its metabolic products. The overloading and rupture of the lysosome can result in autolysis of the hair cell. This may be one of the mechanism of kanamycin ototoxicity.
采用超微细胞化学技术，我们发现所有正常毛细胞中，角质板下方存在高电子密度的溶酶体；但在耳毒性组中，毛细胞内的溶酶体变得多泡状，体积增大并移位。溶酶体的小泡偶尔会融合。在一些毛细胞中，细胞质溶解，细胞膜破裂，细胞内容物泄漏。在这些细胞中，溶酶体消失。我们推测溶酶体中的小泡可能是卡那霉素及其代谢产物。溶酶体的过载和破裂可导致毛细胞自溶。这可能是卡那霉素耳毒性的机制之一。
