Korínková P, Vítek A, Prazák J, Sejkorová J
Ustav hematologie a krevní transfúze, Praha.
Cas Lek Cesk. 1990 Dec 21;129(51):1621-4.
The authors investigated the dependence of the results of the crossover test with thrombocytes and lymphocytes of the donor and serum of the recipient on the rise of thrombocytes 1 and 24 hours following administration of a thrombocyte concentrate. The clinical condition of the patient at the time of thrombocyte administration was taken into account. The donor of thrombocytes isolated in a blood element separator was selected from a panel of cca 800 subjects with previously assessed HLA antigens. A total of 36 administered concentrates was evaluated. From the assembled findings it may be concluded that the result of thromboconcentrate transfusion is significantly influenced by adverse clinical factors in the patient such as splenomegaly, haemorrhage, febrile conditions. A positive crossover lymphocytotoxic test can predict reliably the ineffectiveness of thrombocyte administration. A positive crossover test with the donor's thrombocytes does not affect significantly the success of the transfusion. It seems thus that for the fate of administered thrombocytes the presence of HLA antibodies is decisive and not the presence of specific thrombocytic antibodies.
作者研究了在输注血小板浓缩物后1小时和24小时血小板计数升高情况下，供者血小板和淋巴细胞与受者血清交叉试验结果的相关性。同时考虑了输注血小板时患者的临床状况。在血液成分分离器中分离血小板的供者，是从大约800名先前已评估HLA抗原的受试者中挑选出来的。共评估了36次输注的浓缩物。从汇总结果可以得出结论，患者的不良临床因素如脾肿大、出血、发热状况等对血小板浓缩物输注结果有显著影响。阳性交叉淋巴细胞毒性试验能够可靠地预测血小板输注无效。供者血小板的阳性交叉试验对输血成功与否并无显著影响。因此，对于输注血小板的结局而言，HLA抗体的存在起决定性作用，而非特异性血小板抗体的存在。
