Mooney J L, Hassett D E, Yager L N
Department of Biology, Temple University, Philadelphia, Pennsylvania 19122.
Genetics. 1990 Dec;126(4):869-74. doi: 10.1093/genetics/126.4.869.
Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veA1), which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veA1 that restore the light-dependent conidiation phenotype. Alleles of four genes, defined by complementation tests, were subjected to extensive genetic and phenotypic analysis. The results of light-dark shifting experiments and the phenotypes of double mutant combinations are consistent with the possibility that the expression of the light-dependent phenotype is regulated by specific interactions of the suppressor gene products with the velvet gene product and with each other.
丝状子囊菌构巢曲霉中依赖光的分生孢子形成取决于velvet（veA）基因的等位基因状态。该基因（veA1）中的突变消除了对光的依赖性，使得分生孢子形成能够在无光条件下发生。我们已经分离并鉴定了veA1的六个基因外抑制子，它们恢复了依赖光的分生孢子形成表型。通过互补试验定义的四个基因的等位基因进行了广泛的遗传和表型分析。明暗转换实验的结果以及双突变组合的表型与以下可能性一致，即依赖光的表型的表达是由抑制基因产物与velvet基因产物之间以及它们彼此之间的特定相互作用调节的。
