Theobald Sebastian, Vesth Tammi, Nybo Jane L, Frisvad Jens C, Kjærbølling Inge, Mondo Stephen, LaButti Kurt, Haridas Sajeet, Riley Robert, Kuo Alan A, Salamov Asaf A, Pangilinan Jasmyn, Lipzen Anna, Koriabine Maxim, Yan Mi, Barry Kerrie, Clum Alicia, Lyhne Ellen K, Drula Elodie, Wiebenga Ad, Müller Astrid, Lubbers Ronnie J M, Kun Roland S, Dos Santos Gomes Ana Carolina, Mäkelä Miia R, Henrissat Bernard, Simmons Blake A, Magnuson Jon K, Hoof Jakob B, Mortensen Uffe H, Dyer Paul S, Momany Michelle, Larsen Thomas O, Grigoriev Igor V, Baker Scott E, de Vries Ronald P, Andersen Mikael R
Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Curr Res Microb Sci. 2025 Jan 16;8:100342. doi: 10.1016/j.crmicr.2025.100342. eCollection 2025.
is an important model organism for eukaryotic biology and the reference for the section in comparative studies. In this study, we sequenced the genomes of 25 species of this section. Whole-genome phylogeny of 34 species and clarifies the position of clades inside section . Comparative genomics reveals a high genetic diversity between species with 684 up to 2433 unique protein families. Furthermore, we categorized 2118 secondary metabolite gene clusters (SMGC) into 603 families across Aspergilli, with at least 40 % of the families shared between species. Genetic dereplication of SMGC and subsequent synteny analysis provides evidence for horizontal gene transfer of a SMGC. Proteins that have been investigated in as well as its SMGC families are generally present in the section , supporting its role as model organism. The set of genes encoding plant biomass-related CAZymes is highly conserved in section , while there is remarkable diversity of organization of MAT- both within and between the different clades. This study provides a deeper understanding of the genomic conservation and diversity of this section and supports the position of as a reference species for cell biology.
是真核生物学的重要模式生物，也是比较研究中该类群的参考对象。在本研究中，我们对该类群的25个物种的基因组进行了测序。34个物种的全基因组系统发育分析明确了该类群内各进化枝的位置。比较基因组学揭示了物种间高达684至2433个独特蛋白质家族的高遗传多样性。此外，我们将曲霉菌中的2118个次生代谢物基因簇（SMGC）归类为603个家族，其中至少40%的家族在不同物种间共享。SMGC的遗传去重复及随后的共线性分析为一个SMGC的水平基因转移提供了证据。在该物种及其SMGC家族中研究过的蛋白质通常存在于该类群中，支持了其作为模式生物的作用。编码与植物生物质相关的碳水化合物活性酶（CAZyme）的基因集在该类群中高度保守，而在不同进化枝内部和之间，交配型（MAT）的组织存在显著差异。本研究对该类群的基因组保守性和多样性有了更深入的理解，并支持了该物种作为细胞生物学参考物种的地位。
