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G 蛋白偶联受体介导的葡萄糖感应系统调节光依赖性真菌发育和真菌毒素产生。

GPCR-mediated glucose sensing system regulates light-dependent fungal development and mycotoxin production.

机构信息

Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Ribeirão Preto, Brazil.

Biointeractions and Crop Protection, Rothamsted Research, Hertfordshire, United Kingdom.

出版信息

PLoS Genet. 2019 Oct 14;15(10):e1008419. doi: 10.1371/journal.pgen.1008419. eCollection 2019 Oct.

DOI:10.1371/journal.pgen.1008419
PMID:31609971
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6812930/
Abstract

Microorganisms sense environmental fluctuations in nutrients and light, coordinating their growth and development accordingly. Despite their critical roles in fungi, only a few G-protein coupled receptors (GPCRs) have been characterized. The Aspergillus nidulans genome encodes 86 putative GPCRs. Here, we characterise a carbon starvation-induced GPCR-mediated glucose sensing mechanism in A. nidulans. This includes two class V (gprH and gprI) and one class VII (gprM) GPCRs, which in response to glucose promote cAMP signalling, germination and hyphal growth, while negatively regulating sexual development in a light-dependent manner. We demonstrate that GprH regulates sexual development via influencing VeA activity, a key light-dependent regulator of fungal morphogenesis and secondary metabolism. We show that GprH and GprM are light-independent negative regulators of sterigmatocystin biosynthesis. Additionally, we reveal the epistatic interactions between the three GPCRs in regulating sexual development and sterigmatocystin production. In conclusion, GprH, GprM and GprI constitute a novel carbon starvation-induced glucose sensing mechanism that functions upstream of cAMP-PKA signalling to regulate fungal development and mycotoxin production.

摘要

微生物能够感知环境中营养物质和光照的波动，并相应地协调其生长和发育。尽管它们在真菌中起着关键作用，但只有少数 G 蛋白偶联受体（GPCR）得到了表征。构巢曲霉基因组编码了 86 个假定的 GPCR。在这里，我们描述了构巢曲霉中一种碳饥饿诱导的 GPCR 介导的葡萄糖感应机制。该机制包括两个第五类（gprH 和 gprI）和一个第七类（gprM）GPCR，它们响应葡萄糖促进 cAMP 信号转导、萌发和菌丝生长，同时以光依赖的方式负调控有性生殖。我们证明 GprH 通过影响 VeA 活性来调节有性生殖，VeA 是真菌形态发生和次生代谢的关键光依赖性调节剂。我们表明 GprH 和 GprM 是赤霉素生物合成的光非依赖性负调节剂。此外，我们揭示了这三个 GPCR 在调节有性生殖和麦角甾毒素产生方面的上位性相互作用。总之，GprH、GprM 和 GprI 构成了一种新的碳饥饿诱导的葡萄糖感应机制，该机制在 cAMP-PKA 信号转导的上游起作用，以调节真菌的发育和真菌毒素的产生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/85a34ff30830/pgen.1008419.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/a11054834fcf/pgen.1008419.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/7d554faea67a/pgen.1008419.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/903318128c9b/pgen.1008419.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/6e7d8fad0601/pgen.1008419.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/af1b1e5c70da/pgen.1008419.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/4d9d853e3fa5/pgen.1008419.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/7f8176b8396e/pgen.1008419.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/bdd9553be318/pgen.1008419.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/872017a2d9d8/pgen.1008419.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/85a34ff30830/pgen.1008419.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/a11054834fcf/pgen.1008419.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/7d554faea67a/pgen.1008419.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/903318128c9b/pgen.1008419.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/6e7d8fad0601/pgen.1008419.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/af1b1e5c70da/pgen.1008419.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/4d9d853e3fa5/pgen.1008419.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/7f8176b8396e/pgen.1008419.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/bdd9553be318/pgen.1008419.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/872017a2d9d8/pgen.1008419.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbc7/6812930/85a34ff30830/pgen.1008419.g010.jpg

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