Kuratani S C, Hirano S
Department of Anatomy, Medical College of Georgia, Augusta.
Arch Histol Cytol. 1990 Dec;53(5):575-83. doi: 10.1679/aohc.53.575.
Aberrant ganglion cell morphogenesis associated with the ophthalmic placode was observed within the epidermis of the chick embryo at stages 20 through 26. This process was studied using immunohistochemistry and scanning and transmission electron microscopy. Clusters of ganglion cells were covered by epidermis and seen as epidermal nodules distributed diffusely in the supraorbital region. The ganglion cell clusters found within these nodules were not delineated by basement membrane from the epidermis, and were provisionally termed ectopic ganglia. The cell bodies within the ganglia possessed neurites containing microtubules and neurofilament protein. The cell bodies were also immunoreactive to monoclonal antibodies, E/C8 and HNK-1, indicating neuronal phenotypes. The neurites were often associated with the ophthalmic nerve or its branches as well as being distributed in the ectoderm. The ectopic ganglia seemed to represent the delayed gangliogenesis of a part of the ophthalmic placode which failed to detach from the ectoderm during ophthalmic ganglion formation.
在第20至26阶段的鸡胚表皮内观察到与眼板相关的异常神经节细胞形态发生。使用免疫组织化学、扫描电子显微镜和透射电子显微镜对这一过程进行了研究。神经节细胞簇被表皮覆盖，表现为散在于眶上区域的表皮结节。在这些结节内发现的神经节细胞簇未被基底膜与表皮分隔开，暂称为异位神经节。神经节内的细胞体具有含有微管和神经丝蛋白的神经突。细胞体对单克隆抗体E/C8和HNK-1也具有免疫反应性，表明其具有神经元表型。神经突常与眼神经或其分支相关，也分布在外胚层中。异位神经节似乎代表了眼板一部分的延迟神经节发生，这部分在眼神经节形成过程中未能从外胚层分离。
