Yamada M, Brun G, Weissbach A
J Virol. 1978 May;26(2):281-90. doi: 10.1128/JVI.26.2.281-290.1978.
DNA synthesis in chromatin isolated from herpes simplex virus type 1-infected HeLa cells (HSV chromatin) was examined in vitro. The HSV chromatin was found to carry out an initial limited synthesis of DNA in vitro, 50 to 64 pmol of dTMP incorporated in 10(6) nuclei per 10 min, which is comparable to that found in nuclei isolated from HSV-infected cells. DNA synthesis in vitro proceeded for only 30 min, and both HSV DNA and host DNA were synthesized in significant amounts. The HSV and host DNA synthesis in isolated chromatin were inhibited to the same extent by anti-HSV antiserum or by phosphonoacetic acid. The results indicate that the HSV-induced DNA polymerase is most likely involved in the synthesis of host and HSV DNA in isolated chromatin, even though this chromatin contains small amounts of the host gamma-polymerase in addition to the HSV-induced DNA polymerase. The HSV chromatin contains no detectable levels of DNA polymerases alpha and beta, even though infected cells have normal, or increased, levels of these enzymes.
对从单纯疱疹病毒1型感染的HeLa细胞中分离出的染色质（HSV染色质）的DNA合成进行了体外检测。发现HSV染色质在体外进行初始有限的DNA合成，每10分钟在10⁶个核中掺入50至64皮摩尔的dTMP，这与从HSV感染细胞中分离出的核中的情况相当。体外DNA合成仅持续30分钟，并且大量合成了HSV DNA和宿主DNA。抗HSV抗血清或膦甲酸对分离染色质中的HSV和宿主DNA合成的抑制程度相同。结果表明，HSV诱导的DNA聚合酶很可能参与了分离染色质中宿主和HSV DNA的合成，尽管这种染色质除了含有HSV诱导的DNA聚合酶外还含有少量宿主γ-聚合酶。即使感染细胞中这些酶的水平正常或升高，HSV染色质中也未检测到DNA聚合酶α和β的水平。
