Radsak K, Furukawa T, Plotkin S
Arch Virol. 1980;65(1):45-51. doi: 10.1007/BF01340539.
Chromatin prepared from (14C)-thymidine pulse labelled cytomegalovirus-infected human fibroblasts 72 hours postinfection exhibited under appropriate conditions endogenous activity of (3H)-thymidine triphosphate incorporation which was relatively salt-resistant and phosphonoacetic acid-sensitive. Isopycnic centrifugation of the doubly labelled DNA in CsCl revealed that cell-free incoporation occurred into viral as well as into host cell DNA. Density labelling experiments with bromodeoxyuridine triphosphate suggested the incoporation into viral DNA to be due to replicative DNA synthesis. Chromatin from infected cells contained, in addition to cellular, viral DNA polymerase activity.
从感染巨细胞病毒的人成纤维细胞在感染后72小时用（14C）-胸苷脉冲标记制备的染色质，在适当条件下表现出（3H）-胸苷三磷酸掺入的内源性活性，该活性相对耐盐且对膦乙酸敏感。在CsCl中对双标记DNA进行等密度离心显示，无细胞掺入发生在病毒DNA和宿主细胞DNA中。用溴脱氧尿苷三磷酸进行的密度标记实验表明，掺入病毒DNA是由于复制性DNA合成。来自感染细胞的染色质除了含有细胞DNA聚合酶活性外，还含有病毒DNA聚合酶活性。
