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利用二维电泳和免疫印迹法分析山羊支原体及山羊支原体感染情况

Analysis of caprine mycoplasmas and mycoplasma infections in goats using two-dimensional electrophoresis and immunoblotting.

作者信息

Olsson B, Bölske G, Bergström K, Johansson K E

机构信息

Pharmacia AB, Uppsala, Sweden.

出版信息

Electrophoresis. 1990 Oct;11(10):861-9. doi: 10.1002/elps.1150111016.

DOI:10.1002/elps.1150111016
PMID:2079028
Abstract

Seven strains of mycoplasma have been characterized by two-dimensional polyacrylamide gel electrophoresis in combination with immunoblotting. The two-dimensional patterns of the strains were compared and similarities and differences are discussed. Hyperimmune rabbit antisera were used in the immunoblotting experiment to study the immunological cross-reactivity between the species. Sera from goats, naturally and experimentally infected with mycoplasma, were also used in the immunoblotting experiment to study the immune responses of the infected animals.

摘要

通过二维聚丙烯酰胺凝胶电泳结合免疫印迹法对七株支原体进行了表征。比较了这些菌株的二维图谱，并讨论了它们之间的异同。在免疫印迹实验中使用了超免疫兔抗血清来研究不同种支原体之间的免疫交叉反应性。来自自然感染和实验感染支原体的山羊血清也被用于免疫印迹实验，以研究受感染动物的免疫反应。

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Electrophoresis. 1990 Oct;11(10):861-9. doi: 10.1002/elps.1150111016.
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引用本文的文献

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Acta Vet Scand. 2004;45(3-4):167-79. doi: 10.1186/1751-0147-45-167.
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Genetic and serological analysis of lipoprotein LppA in Mycoplasma mycoides subsp. mycoides LC and Mycoplasma mycoides subsp. capri.蕈状支原体亚种丝状支原体LC株和蕈状支原体亚种山羊支原体中脂蛋白LppA的遗传和血清学分析
Clin Diagn Lab Immunol. 1999 Mar;6(2):224-30. doi: 10.1128/CDLI.6.2.224-230.1999.
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Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes.
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J Bacteriol. 1998 May;180(9):2350-8. doi: 10.1128/JB.180.9.2350-2358.1998.
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Contagious bovine pleuropneumonia in northern Tanzania, culture confirmation and serological studies.坦桑尼亚北部的牛传染性胸膜肺炎，培养确认及血清学研究。
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