Madhyastha H, Vatsala T M
A M M Murugappa Chettiar Research Center, Chennai 600 113, India.
Indian J Exp Biol. 2010 May;48(5):486-93.
Isolation of three different active peptides from C-phycocyanin (C-pc) beta chain of S. fusiformis and their biological properties are reported. Phycocyanin peptide beta fraction 2 (cyanopeptide beta 2) facilitated both antioxidant and plasmid DNA strand scission prevention activity due to higher cysteine moieties in the isolated peptide. The peptide significantly scavenged the free radicals like 1-1,-diphenyl-2-picryl hydrazyl and ferric reducing ability of plasma, increased the absorbance values in reducing power and also showed the higher trolox equivalent antioxidant capacity values in total reactive antioxidant potentials assay. Cyanopeptide beta 2 also inhibited reactive oxygen species induced DNA pBR322 damage in dose dependent manner along with free radical scavenging properties suggesting the role in the DNA integrity which is also evident by DNA binding activity of peptide. In addition, the generation of reactive oxygen species (ROS) was dose dependent (10 and 20 ng/ml) and significantly quenched by cyanopeptide beta2 in human fibroblast cell line TIG 3-20. In vitro cell scratch injury assay demonstrated the capacity of cyanopeptide beta2 in cell migration in to wounded area suggesting fibroblast proliferation and migration. The results suggest that cyanopeptide beta2 can be a free radical scavenger and effective peptide for future biomedical applications like wound healing, atherosclerosis, cell redox potential and hypoxia.
报道了从纺锤藻的C-藻蓝蛋白（C-pc）β链中分离出三种不同的活性肽及其生物学特性。藻蓝蛋白肽β组分2（蓝肽β2）由于分离出的肽中半胱氨酸部分含量较高，具有抗氧化和预防质粒DNA链断裂的活性。该肽能有效清除1,1-二苯基-2-苦基肼自由基和血浆中的铁还原能力，增加还原能力的吸光度值，并且在总活性抗氧化能力测定中显示出较高的Trolox等效抗氧化能力值。蓝肽β2还以剂量依赖的方式抑制活性氧诱导的DNA pBR322损伤，并具有自由基清除特性，这表明其在DNA完整性方面发挥作用，肽的DNA结合活性也证明了这一点。此外，活性氧（ROS）的产生呈剂量依赖性（10和20 ng/ml），并在人成纤维细胞系TIG 3-20中被蓝肽β2显著淬灭。体外细胞划痕损伤试验表明，蓝肽β2具有促进细胞迁移到损伤区域的能力，提示其具有成纤维细胞增殖和迁移作用。结果表明，蓝肽β2可作为自由基清除剂，有望用于伤口愈合、动脉粥样硬化、细胞氧化还原电位和缺氧等未来生物医学应用。
