钝顶螺旋藻 LEB 18 藻蓝蛋白提取物：对脂质体物理化学参数的影响及其与自由基清除/抗氧化性能的相关性。

Spirulina sp. LEB 18-extracted phycocyanin: Effects on liposomes' physicochemical parameters and correlation with antiradical/antioxidant properties.

机构信息

Grupo de Investigação em Interações Moleculares em Membranas, Programa de Pós-Graduação em Química Tecnológica e Ambiental, Brazil; Laboratório de Biotecnologia, Brazil.

Grupo de Investigação em Interações Moleculares em Membranas, Programa de Pós-Graduação em Química Tecnológica e Ambiental, Brazil.

出版信息

Chem Phys Lipids. 2021 May;236:105064. doi: 10.1016/j.chemphyslip.2021.105064. Epub 2021 Feb 17.

DOI:10.1016/j.chemphyslip.2021.105064

PMID:33609502

Abstract

This study describes the physicochemical properties of soybean asolectin (ASO) liposomes loaded with phycocyanin (Phy) extracted from Spirulina sp. LEB 18. The effects of Phy in the liposomes' properties were investigated by Fourier transform infrared spectroscopy (FTIR), H and P nuclear magnetic resonance (NMR), zeta (ζ)-potential, dynamic light scattering (DLS) and ultraviolet-visible (UV-vis) techniques. Phy restricted the motion of ASO polar and interface groups and disrupted the package arrangement of the lipid hydrophobic regions, as a likely effect of dipolar and π interactions related to its amino acid residues and pyrrole portions. These interactions were correlated to antiradical/antioxidant Phy responses obtained by 2,2-diphenyl-1-picrylhidrazil (DPPH) assay, thiobarbituric acid reactive substances (TBARS) and ferric reducing antioxidant power (FRAP) methods, and discussed to bring new chemical perspectives about Phy-loaded liposomes-related nutraceutical applications in inflammatory and viral infection processes.

摘要

本研究描述了负载自噬体藻蓝蛋白（Phy）的大豆溶血磷脂（ASO）脂质体的物理化学性质，该 Phy 是从螺旋藻 LEB 18 中提取的。通过傅里叶变换红外光谱（FTIR）、H 和 P 核磁共振（NMR）、ζ-电位、动态光散射（DLS）和紫外-可见（UV-vis）技术研究了 Phy 对脂质体性质的影响。Phy 限制了 ASO 极性和界面基团的运动，并破坏了脂质疏水区域的包装排列，这可能是由于其氨基酸残基和吡咯部分的偶极和π相互作用所致。这些相互作用与通过 2,2-二苯基-1-苦基肼（DPPH）测定法、硫代巴比妥酸反应物（TBARS）和铁还原抗氧化能力（FRAP）方法获得的 Phy 的抗自由基/抗氧化反应相关，并对其进行了讨论，为 Phy 负载脂质体在炎症和病毒感染过程中的相关营养应用带来了新的化学视角。