Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, 80055 Portici, Italy.
Toxicon. 2010 Dec;56(8):1381-7. doi: 10.1016/j.toxicon.2010.08.003. Epub 2010 Aug 24.
A rapid, high resolution liquid chromatography coupled with ElectroSpray Ionization Time-Of-Flight Mass Spectrometry (ESI/TOF/MS) method was developed for the determination of the toxin pattern in cultured cells of Ostreopsis cf. ovata from the Mediterranean Sea. The samples were separated on a Phenomenex Luna 3μ HILIC 200A (150 × 2.00 mm) and analyzed by LC/TOF/MS with electrospray ionization (ESI) interface in positive ion mode. The method developed here provides the capability for a fully automated analysis, which requires relatively easy sample preparation and gives clean and simple chromatograms. The method was successfully applied to the determination of ovatoxin-a, mascarenotoxin-a and four new palytoxins in O. cf. ovata. Another new palytoxin was detected in the standard material from Palythoa tuberculosa provided by Wako Chemicals.
建立了一种快速、高分辨率的液相色谱-电喷雾电离飞行时间质谱（ESI/TOF/MS）联用方法，用于检测来自地中海的卵形 ostreopsis cf. 培养细胞中的毒素模式。样品在 Phenomenex Luna 3μ HILIC 200A（150×2.00mm）上进行分离，并通过 LC/TOF/MS 在正离子模式下用电喷雾电离（ESI）接口进行分析。这里开发的方法提供了完全自动化分析的能力，需要相对简单的样品制备，并给出干净、简单的色谱图。该方法成功地应用于卵形 ostreopsis cf. 中 ovatoxin-a、mascarenotoxin-a 和四种新的鲍鱼毒素的测定。在 Wako Chemicals 提供的 Palythoa tuberculosa 标准物质中检测到另一种新的鲍鱼毒素。
