Pulsfort Ann K, Wolter Timm P, Pallua Norbert
Department of Plastic and Reconstructive Surgery, Hand Surgery-Burn Center, University Hospital of the RWTH, Pauwelsstrasse 30, Aachen, Germany.
Ann Plast Surg. 2011 Mar;66(3):292-5. doi: 10.1097/SAP.0b013e3181c7140e.
Standard protocols for autologous fat transfer involve centrifugation of the lipoaspirate for better separation. The effect of the centrifugal forces on cell viability and possible induction of apoptosis is discussed controversially in current literature. We have examined the vitality of adipocytes in lipoaspirates after centrifugation by 8 different accelerations up to 20.000g immediately after the respective centrifugation as well as 4 days later by staining. No significant alterations in the viability of differently centrifuged adipocytes were found. Cultivation of the samples after centrifugation revealed no apoptotic changes. Our results show that centrifugation, as a part of the protocol of lipofilling, has no effect on the survival rate of isolated adipocytes in the purified fat. In contrast, lipoaspirates centrifuged with higher accelerations seem to be better cleansed of oil and cell debris than samples treated with lower centrifugal forces.
自体脂肪移植的标准方案包括对脂肪抽吸物进行离心，以实现更好的分离。目前的文献中，对于离心力对细胞活力及可能诱导细胞凋亡的影响存在争议。我们通过8种不同加速度（最高达20,000g）对脂肪抽吸物进行离心，并在离心后立即以及4天后通过染色检查脂肪细胞的活力。结果发现，不同离心条件下的脂肪细胞活力无显著变化。离心后对样本进行培养，未发现凋亡变化。我们的结果表明，作为脂肪填充方案的一部分，离心对纯化脂肪中分离出的脂肪细胞存活率没有影响。相比之下，与采用较低离心力处理的样本相比，以较高加速度离心的脂肪抽吸物似乎能更好地清除油脂和细胞碎片。
