Department of Orthopedic, Traumatologic, Rehabilitative e Plastic-Reconstructive, Second University of Naples, L. De Crecchio 3, Naples, Italy.
Aesthetic Plast Surg. 2011 Jun;35(3):341-8. doi: 10.1007/s00266-010-9613-8. Epub 2010 Nov 11.
The use of adipose tissue transfer in plastic and reconstructive surgery is not new and has been studied extensively. Due to different results with regard to adipose cell damage and the level of survival of the transferred tissue in clinical practice, the authors aimed to investigate the effects of centrifugation on fat aspirates to optimize the centrifugal force for fat transplantation and to obtain an increased number of intact adipose progenitor cells. The following different centrifugation forces were evaluated in vitro in terms of fat decantation: 3,000 rpm (1,500×g), 1,300 rpm (250×g), and 500 rpm (50×g). Moreover, the density level, morphology of fat cells, cell viability, and progenitor cell number also were evaluated. Centrifugation leads to a good fat tissue density, with a significant number of progenitor cells, and efficiently removes the liquid portion. High centrifugal forces (at 3,000 rpm) caused significant damage to fat cells with low cell viability, whereas very low centrifugal forces (at 500 rpm) showed little effect on adipose tissue density, resembling fat decantation. Fat aspirates, withdrawn from 30 healthy donors in vivo, were centrifuged at different rotations per minute (rpm), as follows. For the 10 patients in group A, Coleman's technique was used with a centrifugation of the aspirated fat at 3,000 rpm (1,500×g) for 3 min. For the 10 patients in group B, the authors' technique was used, with centrifugation of the aspirated fat at 1,300 rpm (250×g) for 5 min. For the 10 patients in group C, simple decantation of fat was used. In conclusion, a centrifugal force of 1,300 rpm resulted in better density of adipose tissue, with good cell viability and increased ability to preserve a significant number of progenitor cells.
脂肪组织移植在整形和重建外科中并不新鲜，已经进行了广泛的研究。由于在临床实践中脂肪细胞损伤和移植组织存活率方面的结果不同，作者旨在研究离心对脂肪抽吸物的影响，以优化脂肪移植的离心力，并获得更多数量的完整脂肪祖细胞。在体外，我们评估了以下不同的离心力对脂肪沉降的影响：3000rpm（1500×g）、1300rpm（250×g）和 500rpm（50×g）。此外，还评估了脂肪细胞的密度水平、形态、细胞活力和祖细胞数量。离心导致脂肪组织的良好密度，有大量的祖细胞，并且有效地去除了液体部分。高离心力（在 3000rpm 时）导致脂肪细胞严重损伤，细胞活力低，而非常低的离心力（在 500rpm 时）对脂肪组织密度几乎没有影响，类似于脂肪沉降。从 30 名体内健康供体中抽取的脂肪抽吸物，在不同每分钟转数（rpm）下进行离心，如下所示。对于 A 组的 10 名患者，采用科尔曼技术，将抽吸的脂肪在 3000rpm（1500×g）下离心 3 分钟。对于 B 组的 10 名患者，采用作者的技术，将抽吸的脂肪在 1300rpm（250×g）下离心 5 分钟。对于 C 组的 10 名患者，仅进行简单的脂肪沉降。总之，1300rpm 的离心力可使脂肪组织的密度更好，细胞活力良好，并能更好地保留大量祖细胞。
