Department of Plastic & Reconstructive Surgery, Shanghai Ninth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 639 Zhizhaoju Road, Shanghai 200011, PR China.
J Plast Reconstr Aesthet Surg. 2010 Mar;63(3):482-7. doi: 10.1016/j.bjps.2008.11.056. Epub 2009 Jan 6.
An up-to-date, simple, but useful technique to evaluate the viability of fat grafts prior to transplant is lacking. The purpose of this study is to introduce the glucose transport test - a new method to evaluate the viability of fat grafts after they are subjected to different centrifugal forces in vitro.
Fat grafts were harvested from healthy patients who underwent liposuction for body contouring. The glucose transport test was performed to evaluate the viability of fat grafts after centrifugation with different forces (1000-4000 rpm). An MTT assay was also performed with the same experimental protocol for comparison. Routine histological examination was done in all groups to examine possible structural destruction after centrifugation.
When compared with the group not subjected to centrifugation, the glucose transport test showed a significant decrease in viability of fat grafts in all of the other four groups (all p<0.001). There was a linear reduction of viability in fat grafts with the increase in centrifugal force (all p<0.03). MTT assay showed similar findings on the viability of fat grafts in all five groups and correlated well with the glucose transport test (r=0.9870). Histology showed significantly distorted and fractured adipocytes when the centrifugal force reached 4000 rpm.
Our study demonstrates the harmful effect on the viability of fat grafts with an increase in centrifugal force and, for the first time, that the glucose transport test may be an effective and potentially useful method to evaluate the viability of fat grafts in a clinical setting.
目前缺乏一种能够在移植前评估脂肪移植物活力的、最新的、简单但实用的技术。本研究旨在介绍葡萄糖转运测试，这是一种在体外对不同离心力作用下的脂肪移植物的活力进行评估的新方法。
从接受吸脂术以塑造身体轮廓的健康患者中采集脂肪移植物。进行葡萄糖转运测试以评估在不同离心力（1000-4000 rpm）下离心后脂肪移植物的活力。还使用相同的实验方案进行 MTT 测定以进行比较。对所有组均进行常规组织学检查，以检查离心后可能发生的结构破坏。
与未进行离心的组相比，葡萄糖转运测试显示所有其他四组的脂肪移植物活力均显著降低（均 P<0.001）。随着离心力的增加，脂肪移植物的活力呈线性下降（均 P<0.03）。MTT 测定在所有五组的脂肪移植物活力上均显示出相似的发现，与葡萄糖转运测试相关性良好（r=0.9870）。组织学检查显示当离心力达到 4000 rpm 时，脂肪细胞明显变形和断裂。
我们的研究表明，随着离心力的增加，脂肪移植物的活力会受到有害影响，并且首次表明葡萄糖转运测试可能是一种在临床环境中评估脂肪移植物活力的有效且有潜在用途的方法。
