Chinese Academy of Inspection and Quarantine, NO.241, Huixinxijie, Chaoyang district, Beijing, P. R. China.
J Microbiol. 2010 Aug;48(4):480-5. doi: 10.1007/s12275-010-9301-z. Epub 2010 Aug 20.
Carbonyl sulfide (COS) is a new fumigant used in phytosanitary treatments. It was developed as a potential alternative to methyl bromide, which is being phased out because of its ozone-depletion properties. To understand the molecular and cellular mechanisms occurring in fungal pathogens in response to COS fumigation, we cloned 510 cDNA fragments of Alternaria alternata (Fr.) Keissler genes that are differentially expressed; these genes were cloned using suppression subtractive hybridization. Changes in the levels of transcripts of 79 fragments were confirmed by microarray analysis and qRT-PCR. Further homology search revealed that they are highly homologous to 41 genes of other fungi, which were related to general metabolism, growth and division, defense, cellular transport, and signal transduction. These results provide an overview of differential expression profiles of A. alternata genes following COS treatment and some new clues about the mechanism of COS fungitoxicity.
羰基硫 (COS) 是一种新的植物检疫熏蒸剂，被开发出来作为正在逐步淘汰的溴甲烷的替代品，因其具有破坏臭氧层的特性。为了了解真菌病原体在受到 COS 熏蒸时发生的分子和细胞机制，我们克隆了Alternaria alternata (Fr.) Keissler 基因的 510 个差异表达 cDNA 片段；这些基因是使用抑制性消减杂交克隆的。微阵列分析和 qRT-PCR 证实了 79 个片段的转录水平变化。进一步的同源性搜索表明，它们与其他真菌的 41 个基因高度同源，这些基因与一般代谢、生长和分裂、防御、细胞运输和信号转导有关。这些结果提供了 A. alternata 基因在 COS 处理后的差异表达图谱概述，并为 COS 杀菌毒性机制提供了一些新的线索。
