Institute of Public Health, Medical School, University of Pécs, Szigeti str. 12, H-7643 Pécs, Hungary.
Phytother Res. 2011 Apr;25(4):493-500. doi: 10.1002/ptr.3281. Epub 2010 Aug 26.
A long-term experimental animal model was developed by our research group for the evaluation of potential chemopreventive effects. The inhibitory effects of agents on carcinogen (7,12-dimethylbenz[a]anthracene (DMBA) induced molecular epidemiological biomarkers, in this case the expression of key onco/suppressor genes were investigated. The expression pattern of c-myc, Ha-ras, Bcl-2, K-ras protooncogene and p53 tumour suppressor gene were studied to elucidate early carcinogenic and potential chemopreventive effects. The consumption of so-called Claw of Dragon tea (CoD™ tea) containing the bark of Uncaria guianensis, Cat's Claw (Uncaria sp. U. tomentosa) and Palmer trumpet-tree (Tabebuia sp. T. avellanedae) was able to decrease the DMBA-induced onco/suppressor gene overexpression in a short-term animal experiment. In a following study CBA/Ca mice were treated with 20 mg/kg bw DMBA intraperitoneally (i.p.) and the expression patterns of onco/suppressor genes were examined at several time intervals. According to the examined gene expression patterns in this long-term experiment the chemopreventive effect of CoD™ tea consumption could be confirmed.
我们的研究小组开发了一种长期的实验动物模型，用于评估潜在的化学预防作用。研究了药物对致癌物（7,12-二甲基苯并[a]蒽（DMBA）诱导的分子流行病学生物标志物的抑制作用，在这种情况下，研究了关键癌基因/抑癌基因的表达。研究了 c-myc、Ha-ras、Bcl-2、K-ras 原癌基因和 p53 肿瘤抑制基因的表达模式，以阐明早期致癌作用和潜在的化学预防作用。所谓的爪龙茶（CoD™茶）的消费，其中含有钩藤 Uncaria guianensis、猫爪 Uncaria sp. U. tomentosa 和帕默喇叭树 Tabebuia sp. T. avellanedae 的树皮，能够减少短期动物实验中 DMBA 诱导的癌基因/抑癌基因过表达。在随后的研究中，CBA/Ca 小鼠用 20mg/kg bw DMBA 腹腔内（i.p.）处理，并在几个时间间隔检查癌基因/抑癌基因的表达模式。根据这项长期实验中检查的基因表达模式，证实了 CoD™茶消费的化学预防作用。
