J Biomed Opt. 2010 Jul-Aug;15(4):040503. doi: 10.1117/1.3467495.
A fully integrated trimodality fluorescence, diffuse optical, and x-ray computed tomography (FT/DOT/XCT) system for small animal imaging is reported in this work. The main purpose of this system is to obtain quantitatively accurate fluorescence concentration images using a multimodality approach. XCT offers anatomical information, while DOT provides the necessary background optical property map to improve FT image accuracy. The quantitative accuracy of this trimodality system is demonstrated in vivo. In particular, we show that a 2-mm-diam fluorescence inclusion located 8 mm deep in a nude mouse can only be localized when functional a priori information from DOT is available. However, the error in the recovered fluorophore concentration is nearly 87%. On the other hand, the fluorophore concentration can be accurately recovered within 2% error when both DOT functional and XCT structural a priori information are utilized together to guide and constrain the FT reconstruction algorithm.
本文报道了一种用于小动物成像的完全集成的荧光、漫射光学和 X 射线计算机断层扫描（FT/DOT/XCT）系统。该系统的主要目的是使用多模态方法获得定量准确的荧光浓度图像。XCT 提供解剖信息，而 DOT 提供必要的背景光学特性图以提高 FT 图像的准确性。该三模态系统的定量准确性在体内得到了验证。具体来说，我们表明，当可利用来自 DOT 的功能先验信息时，只有位于裸鼠 8 毫米深处的 2 毫米直径荧光包含物才能被定位。然而，恢复的荧光团浓度的误差接近 87%。另一方面，当同时利用 DOT 功能和 XCT 结构先验信息来指导和约束 FT 重建算法时，荧光团浓度可以准确地以 2%的误差恢复。
