University of California, Tu and Yuen Center for Functional Onco-Imaging, Department of Radiological Sciences, 164 Irvine Hall, Irvine, California 92697, USA.
J Biomed Opt. 2012 Dec;17(12):126021. doi: 10.1117/1.jbo.17.12.126021.
We have developed a hybrid frequency domain fluorescence tomography and magnetic resonance imaging system (MRI) for small animal imaging. The main purpose of this system is to obtain quantitatively accurate fluorescence concentration and lifetime images using a multi-modality approach. In vivo experiments are undertaken to evaluate the system. We compare the recovered fluorescence parameters with and without MRI structural a priori information. In addition, we compare two optical background heterogeneity correction methods: Born normalization and utilizing diffuse optical tomography (DOT) functional a priori information. The results show that the concentration and lifetime of a 4.2-mm diameter indocyanine green inclusion located 15 mm deep inside a rat can be recovered with less than a 5% error when functional a priori information from DOT and structural a priori information from MRI are utilized.
我们开发了一种用于小动物成像的混合频域荧光层析成像和磁共振成像系统（MRI）。该系统的主要目的是使用多模态方法获得定量准确的荧光浓度和寿命图像。进行了体内实验来评估该系统。我们比较了有无 MRI 结构先验信息时恢复的荧光参数。此外，我们还比较了两种光学背景不均匀性校正方法：Born 归一化和利用漫射光学层析成像（DOT）功能先验信息。结果表明，当利用 DOT 的功能先验信息和 MRI 的结构先验信息时，位于大鼠 15mm 深处的直径为 4.2mm 的吲哚菁绿包含物的浓度和寿命可以恢复到小于 5%的误差。
