Sutton Elizabeth J, Henning Tobias D, Pichler Bernd J, Bremer Christoph, Daldrup-Link Heike E
Department of Radiology, University of California, San Francisco, CA 94143-0628, USA.
Eur Radiol. 2008 Oct;18(10):2021-32. doi: 10.1007/s00330-008-0984-z. Epub 2008 May 28.
Adaptability, sensitivity, resolution and non-invasiveness are the attributes that have contributed to the longstanding use of light as an investigational tool and form the basis of optical imaging (OI). OI, which encompasses numerous techniques and methods, is rapid (<5 min), inexpensive, noninvasive, nontoxic (no radiation) and has molecular (single-cell) sensitivity, which is equal to that of conventional nuclear imaging and several orders of magnitude greater than MRI. This article provides a comprehensive overview of emerging applications of OI-based techniques for in vivo monitoring of new stem cell-based therapies. Different fluorochromes for cell labeling, labeling methods and OI-based cell-tracking techniques will be reviewed with respect to their technical principles, current applications and aims for clinical translation. Advantages and limitations of these new OI-based cell-tracking techniques will be discussed. Non-invasive mapping of cells labeled with fluorochromes or OI marker genes has the potential to evolve further within the clinical realm.
适应性、敏感性、分辨率和非侵入性是长期以来光作为一种研究工具所具备的特性，也是光学成像（OI）的基础。OI涵盖众多技术和方法，具有快速（<5分钟）、廉价、非侵入性、无毒（无辐射）以及分子（单细胞）敏感性的特点，其敏感性与传统核成像相当，比MRI高出几个数量级。本文全面概述了基于OI的技术在体内监测新型干细胞疗法方面的新兴应用。将针对不同的细胞标记荧光染料、标记方法以及基于OI的细胞追踪技术，就其技术原理、当前应用和临床转化目标进行综述。还将讨论这些新型基于OI的细胞追踪技术的优缺点。用荧光染料或OI标记基因标记的细胞的非侵入性图谱绘制在临床领域有进一步发展的潜力。
