基于透射电子显微镜的液泡ATP酶结构模型。

A structural model of the vacuolar ATPase from transmission electron microscopy.

作者信息

Wilkens Stephan, Zhang Zhenyu, Zheng Yesha

机构信息

Department of Biochemistry, University of California, Riverside, CA 92521, USA.

出版信息

Micron. 2005;36(2):109-26. doi: 10.1016/j.micron.2004.10.002.

DOI:10.1016/j.micron.2004.10.002

PMID:15629643

Abstract

Vacuolar ATPases (V-ATPases) are large, membrane bound, multisubunit protein complexes which function as ATP hydrolysis driven proton pumps. V-ATPases and related enzymes are found in the endomembrane system of eukaryotic organsims, the plasma membrane of specialized cells in higher eukaryotes, and the plasma membrane of prokaryotes. The proton pumping action of the vacuolar ATPase is involved in a variety of vital intra- and inter-cellular processes such as receptor mediated endocytosis, protein trafficking, active transport of metabolites, homeostasis and neurotransmitter release. This review summarizes recent progress in the structure determination of the vacuolar ATPase focusing on studies by transmission electron microscopy. A model of the subunit architecture of the vacuolar ATPase is presented which is based on the electron microscopic images and the available information from genetic, biochemical and biophysical experiments.

摘要

液泡型ATP酶（V-ATP酶）是大型的、膜结合的多亚基蛋白质复合物，其功能是作为ATP水解驱动的质子泵。V-ATP酶及相关酶存在于真核生物的内膜系统、高等真核生物特化细胞的质膜以及原核生物的质膜中。液泡型ATP酶的质子泵作用参与多种重要的细胞内和细胞间过程，如受体介导的内吞作用、蛋白质运输、代谢物的主动运输、内环境稳定和神经递质释放。本综述总结了液泡型ATP酶结构测定的最新进展，重点是透射电子显微镜研究。基于电子显微镜图像以及遗传、生化和生物物理实验的现有信息，提出了液泡型ATP酶亚基结构模型。