College of Life Science, Shandong Agricultural University, Daizong Street 61, Taian, Shandong 271018, PR China.
J Biosci Bioeng. 2011 Jan;111(1):50-4. doi: 10.1016/j.jbiosc.2010.08.004.
Response surface methodology (RSM) was used to optimize the extraction parameters for Pholiota adiposa SX-02 intracellular polysaccharide (IPS) produced during submerged culture. The optimum conditions of IPS extraction were predicted to be, ultrasonic power at 564.93 W, precipitation time 30.34 h and pH 8.28, and IPS yield was estimated at 19.75%. The actual value of IPS under these conditions was 20.51%. The in vitro antioxidant results showed that the inhibition effects of IPS at a dosage of 250 mg/l on superoxide anion, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were 74.66 ± 5.31%, 69.20 ± 5.13%, and 75.20 ± 6.73%, respectively, which were 27.51 ± 2.23%, 16.58 ± 1.33%, and 9.46 ± 0.72% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.32 ± 0.02 (absorbance at 700 nm), 39.13 ± 3.47% higher than that of BHT. The results provide a reference for large-scale extraction of IPS by P. adiposa SX-02 in industrial fermentation and the IPS can be used as a potential antioxidant which enhances adaptive immune responses.
响应面法（RSM）用于优化 Pholiota adiposa SX-02 胞内多糖（IPS）的浸提工艺参数。预测 IPS 提取的最佳条件为超声功率 564.93 W、沉淀时间 30.34 h、pH 值 8.28，IPS 得率为 19.75%。在这些条件下 IPS 的实际值为 20.51%。体外抗氧化结果表明，在 250 mg/L 剂量下，IPS 对超氧阴离子、羟基和 1,1-二苯基-2-苦基肼（DPPH）自由基的抑制率分别为 74.66 ± 5.31%、69.20 ± 5.13%和 75.20 ± 6.73%，分别比丁基羟基甲苯（BHT）高 27.51 ± 2.23%、16.58 ± 1.33%和 9.46 ± 0.72%。IPS 的还原力为 0.32 ± 0.02（700nm 处吸光度），比 BHT 高 39.13 ± 3.47%。结果为 P. adiposa SX-02 在工业发酵中大规模提取 IPS 提供了参考，同时 IPS 可作为一种潜在的抗氧化剂，增强适应性免疫反应。
