Center for Mitochondrial Disease, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
J Gerontol A Biol Sci Med Sci. 2010 Nov;65(11):1157-64. doi: 10.1093/gerona/glq141. Epub 2010 Aug 26.
We measured the loss of cardiac mitochondrial function related to aging in males of three rat strains presenting with different longevity and aging phenotypes: the Fischer 344 (F344), the Brown Norway (BN), and the hybrid F344×BN. The F344 rat has a short life span and a ∼45% decrease in coupled mitochondrial oxidation in the cardiac permeabilized fibers from the old rats compared with the young rats. Citrate synthase activity in the permeabilized fibers (mitochondrial content) did not change significantly with aging. The BN live longer compared with the F344 and have a 15%-18% loss of mitochondrial respiration in the aged rats compared with the young rats. The differences are not significant. In hybrids, more resistant to aging than are the BN and the F344, mitochondrial function is preserved during aging. The difference in longevity of the different strains is correlated with mitochondrial dysfunction in the heart, suggesting the importance of mitochondria in cardiac aging.
我们测量了与衰老相关的雄性三种大鼠品系（具有不同长寿和衰老表型的 Fischer 344（F344）、Brown Norway（BN）和 F344×BN 杂交品系）中心脏线粒体功能的丧失。F344 大鼠寿命较短，与年轻大鼠相比，老年大鼠心脏通透纤维中偶联线粒体氧化的能力下降了约 45%。通透纤维中柠檬酸合酶的活性（线粒体含量）随老化变化不明显。BN 大鼠的寿命比 F344 长，与年轻大鼠相比，老年大鼠的线粒体呼吸丧失了 15%-18%。这些差异并不显著。在杂种大鼠中，它们比 BN 和 F344 更能抵抗衰老，线粒体功能在衰老过程中得以维持。不同品系之间的寿命差异与心脏中线粒体功能障碍有关，这表明线粒体在心脏衰老中的重要性。
