School of Civil and Environmental Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Appl Environ Microbiol. 2010 Oct;76(20):6947-50. doi: 10.1128/AEM.01095-10. Epub 2010 Aug 27.
The occurrence of 10 pathogens and three fecal indicators was assessed by quantitative PCR in manures of Australian feedlot cattle. Most samples tested positive for one or more pathogens. For the dominant pathogens Campylobacter jejuni, Listeria monocytogenes, Giardia spp., Cryptosporidium spp., and eaeA-positive Escherichia coli, 10² to 10⁷ genome copies g⁻¹ (dry weight) manure were recovered.
采用定量 PCR 方法对澳大利亚饲养场牛的粪便中 10 种病原体和 3 种粪便指示菌进行了检测。大多数样品检测呈一种或多种病原体阳性。对于占主导地位的病原体空肠弯曲菌、单核细胞增生李斯特菌、贾第虫属、隐孢子虫属和 eaeA 阳性大肠杆菌,每克(干重)粪便中回收了 10² 到 10⁷ 基因组拷贝。