Filosa Jessica A
Department of Physiology, Medical College of Georgia Augusta, GA, USA.
Front Neuroenergetics. 2010 Aug 16;2. doi: 10.3389/fnene.2010.00016. eCollection 2010.
Neurovascular research has made significant strides toward understanding how the brain neurovascular unit accomplishes rapid and spatial increases in blood flow following neuronal activation. Among the experimental models used, the in vitro brain slice preparation provides unique information revealing the potential signals and cellular mechanisms involved in functional hyperemia. The most crucial limitation of this model, however, is the lack of intraluminal pressure and flow in the vessels being studied. Moreover, differences in basal vascular tone have led to varied interpretations regarding the polarity of vascular responses following neuron-to-glial stimulation. Given the complexity of astrocyte-induced neurovascular responses, we propose the use of a modified in vitro brain slice preparation, where intraluminal arteriolar pressure and flow are retained. Throughout this review, we discuss the advantages and disadvantages to be considered when using brain slices for neurovascular studies. Potential ways to overcome the current limitations are proposed.
神经血管研究在理解大脑神经血管单元如何在神经元激活后实现血流的快速和空间增加方面取得了重大进展。在使用的实验模型中，体外脑片制备提供了独特的信息，揭示了参与功能性充血的潜在信号和细胞机制。然而，该模型最关键的局限性在于所研究血管中缺乏腔内压力和血流。此外，基础血管张力的差异导致了对神经元到胶质细胞刺激后血管反应极性的不同解释。鉴于星形胶质细胞诱导的神经血管反应的复杂性，我们建议使用一种改良的体外脑片制备方法，该方法保留腔内小动脉压力和血流。在这篇综述中，我们讨论了在使用脑片进行神经血管研究时需要考虑的优缺点。还提出了克服当前局限性的潜在方法。
