Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
Electrophoresis. 2010 Sep;31(18):3152-8. doi: 10.1002/elps.201000212.
This paper reports the development of a novel force measurement device based on polymeric nanopillar arrays. The device was fabricated by a process combining nanosphere lithography, oxygen plasma treatment, deep etching and nano-molding. Well-ordered polymeric nanopillar arrays with various diameters and aspect ratios were fabricated and used as cell culture substrates. Cell traction forces were measured by the deflection of the nanopillars. Since the location of the nanopillars can be monitored at all times, this device allows for the measurement of the evolution of adhesion forces over time.
本文报道了一种基于聚合物纳米柱阵列的新型力测量装置的研制。该装置通过结合纳米球光刻、氧等离子体处理、深蚀刻和纳米成型工艺制造而成。制备了具有不同直径和纵横比的有序聚合物纳米柱阵列，并将其用作细胞培养基底。通过纳米柱的挠度来测量细胞牵引力。由于可以随时监测纳米柱的位置，因此该装置可以测量随时间变化的粘附力的演变。
