Hospital Epidemiology, University of North Carolina Health Care, and the Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7030, USA.
Infect Control Hosp Epidemiol. 2010 Oct;31(10):1025-9. doi: 10.1086/656244.
To determine the effectiveness of a UV-C-emitting device to eliminate clinically important nosocomial pathogens in a contaminated hospital room.
This study was carried out in a standard but empty hospital room (phase 1) and in a room previously occupied by a patient with methicillin-resistant Staphylococcus aureus (MRSA) or vancomycin-resistant Enterococcus (VRE) infection (phase 2) in an acute care tertiary hospital in North Carolina from January 21 through September 21, 2009. During phase 1, 8 x 8 cm Formica sheets contaminated with approximately 10(4)-10(5) organisms of MRSA, VRE, multidrug-resistant (MDR) Acinetobacter baumannii, or Clostridium difficile spores were placed in a hospital room, both in direct line of sight of the UV-C device and behind objects. After timed exposure, the presence of the microbes was assessed. During phase 2, specific sites in rooms that had housed patients with MRSA or VRE infection were sampled before and after UV-C irradiation. After timed exposure, the presence of MRSA and VRE and total colony counts were assessed.
In our test room, the effectiveness of UV-C radiation in reducing the counts of vegetative bacteria on surfaces was more than 99.9% within 15 minutes, and the reduction in C. difficile spores was 99.8% within 50 minutes. In rooms occupied by patients with MRSA, UV-C irradiation of approximately 15 minutes duration resulted in a decrease in total CFUs per plate (mean, 384 CFUs vs 19 CFUs; P < .001), in the number of samples positive for MRSA (81 [20.3%] of 400 plates vs 2 [0.5%] of 400 plates; P < .001), and in MRSA counts per MRSA-positive plate (mean, 37 CFUs vs 2 CFUs; P < .001).
This UV-C device was effective in eliminating vegetative bacteria on contaminated surfaces both in the line of sight and behind objects within approximately 15 minutes and in eliminating C. difficile spores within 50 minutes.
确定紫外线（UV）-C 发射装置在受污染的医院病房中消除临床重要医院病原体的效果。
这项研究于 2009 年 1 月 21 日至 9 月 21 日在北卡罗来纳州的一家急性护理三级医院进行，研究在一间标准但空的医院病房（第 1 阶段）和一间先前被耐甲氧西林金黄色葡萄球菌（MRSA）或万古霉素耐药肠球菌（VRE）感染的患者占用的病房（第 2 阶段）进行。在第 1 阶段，将大约 10（4）-10（5）个 MRSA、VRE、多药耐药（MDR）鲍曼不动杆菌或艰难梭菌孢子的 8 x 8 cm 厚的富美家（Formica）片放置在医院病房中，既在 UV-C 装置的直接视线内，也在视线后。经过定时暴露后，评估微生物的存在情况。在第 2 阶段，在 MRSA 或 VRE 感染患者居住过的房间中，在 UV-C 照射前后采集特定部位的样本。经过定时暴露后，评估 MRSA 和 VRE 的存在情况以及总菌落计数。
在我们的测试室中，UV-C 辐射在 15 分钟内使表面上的繁殖细菌计数减少超过 99.9%，而艰难梭菌孢子的减少则为 99.8%，用时 50 分钟。在 MRSA 患者居住的房间中，持续约 15 分钟的 UV-C 照射导致每个平板的总 CFU 减少（平均值，384 CFU 对 19 CFU；P <.001），MRSA 阳性样本数量减少（81 [20.3%] 对 400 个平板，2 [0.5%] 对 400 个平板；P <.001），以及每个 MRSA 阳性平板的 MRSA 计数减少（平均值，37 CFU 对 2 CFU；P <.001）。
该 UV-C 装置在大约 15 分钟内有效消除视线内和视线后受污染表面上的繁殖细菌，并在 50 分钟内消除艰难梭菌孢子。
