Nerandzic Michelle M, Thota Priyaleela, Sankar C Thriveen, Jencson Annette, Cadnum Jennifer L, Ray Amy J, Salata Robert A, Watkins Richard R, Donskey Curtis J
1Research Service,Louis Stokes Cleveland Veterans Affairs Medical Center,Cleveland,Ohio.
2Case Western Reserve University School of Medicine,Cleveland,Ohio.
Infect Control Hosp Epidemiol. 2015 Feb;36(2):192-7. doi: 10.1017/ice.2014.36.
OBJECTIVE To determine the effectiveness of a pulsed xenon ultraviolet (PX-UV) disinfection device for reduction in recovery of healthcare-associated pathogens. SETTING Two acute-care hospitals. METHODS We examined the effectiveness of PX-UV for killing of Clostridium difficile spores, methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococcus (VRE) on glass carriers and evaluated the impact of pathogen concentration, distance from the device, organic load, and shading from the direct field of radiation on killing efficacy. We compared the effectiveness of PX-UV and ultraviolet-C (UV-C) irradiation, each delivered for 10 minutes at 4 feet. In hospital rooms, the frequency of native pathogen contamination on high-touch surfaces was assessed before and after 10 minutes of PX-UV irradiation. RESULTS On carriers, irradiation delivered for 10 minutes at 4 feet from the PX-UV device reduced recovery of C. difficile spores, MRSA, and VRE by 0.55±0.34, 1.85±0.49, and 0.6±0.25 log10 colony-forming units (CFU)/cm2, respectively. Increasing distance from the PX-UV device dramatically reduced killing efficacy, whereas pathogen concentration, organic load, and shading did not. Continuous UV-C achieved significantly greater log10CFU reductions than PX-UV irradiation on glass carriers. On frequently touched surfaces, PX-UV significantly reduced the frequency of positive C. difficile, VRE, and MRSA culture results. CONCLUSIONS The PX-UV device reduced recovery of MRSA, C. difficile, and VRE on glass carriers and on frequently touched surfaces in hospital rooms with a 10-minute UV exposure time. PX-UV was not more effective than continuous UV-C in reducing pathogen recovery on glass slides, suggesting that both forms of UV have some effectiveness at relatively short exposure times.
目的 确定脉冲氙气紫外线（PX-UV）消毒设备在减少医疗相关病原体复苏方面的有效性。地点 两家急症护理医院。方法 我们检测了PX-UV对玻璃载体上艰难梭菌孢子、耐甲氧西林金黄色葡萄球菌（MRSA）和耐万古霉素肠球菌（VRE）的杀灭效果，并评估了病原体浓度、与设备的距离、有机负荷以及来自直接辐射场的遮蔽对杀灭效果的影响。我们比较了PX-UV和紫外线-C（UV-C）照射（均在4英尺处照射10分钟）的有效性。在医院病房中，评估了PX-UV照射10分钟前后高接触表面上天然病原体污染的频率。结果 在载体上，距PX-UV设备4英尺处照射10分钟可使艰难梭菌孢子、MRSA和VRE的复苏分别降低0.55±0.34、1.85±0.49和0.6±0.25 log10菌落形成单位（CFU）/cm²。与PX-UV设备的距离增加会显著降低杀灭效果，而病原体浓度、有机负荷和遮蔽则不会。在玻璃载体上，连续UV-C实现的log10CFU降低显著大于PX-UV照射。在经常接触的表面上，PX-UV显著降低了艰难梭菌、VRE和MRSA阳性培养结果的频率。结论 PX-UV设备在紫外线照射10分钟的情况下，可降低医院病房玻璃载体和经常接触表面上MRSA、艰难梭菌和VRE的复苏。在减少载玻片上的病原体复苏方面，PX-UV并不比连续UV-C更有效，这表明两种形式的紫外线在相对较短的暴露时间内都有一定效果。
