Hande Alka Harish, Chaudhary Minal S
Department of Oral and Maxillofacial Pathology, Sharad Pawar Dental College, Meghe, Wardha, India.
Rom J Morphol Embryol. 2010;51(3):527-32.
The objective of the present study was to assess the effect of tobacco chewing on buccal mucosa by using cytomorphometry.
We compared the cellular diameter (CD), nuclear diameter (ND) and the ratio of nuclear diameter to cellular diameter (N/C) of buccal mucosa squames of normal subjects (N) with buccal mucosa squames of tobacco users without lesion (A), with tobacco-lime lesion (B), leukoplakia (C), and oral squamous cell carcinoma (D). The study group consisted of 125 patients divided into five groups (N, A, B, C and D) between the ages of 21 and 75 years.
The mean of the cellular diameter (in micrometers) of group N, A, B, C, and D was 72.86+/-5.26, 68.30+/-3.02, 62.13+/-3.29, 57.75+/-4.66, 54.51+/-4.66 respectively (p<0.01). The mean of the nuclear diameter (in micrometers) of group N, A, B, C, and D was 8.70+/-1.30, 8.98+/-1.08, 9.06+/-0.83, 9.12+/-1.06, and 11.04+/-1.46 respectively (p<0.01). The mean of the ratio of nuclear diameter to cellular diameter of group N, A, B, C, and D was 0.11+/-2.00, 0.13+/-1.82, 0.14+/-1.35, 0.16+/-3.11, 0.21+/-4.51 respectively (p<0.01). Univariate analysis of variance (ANOVA) showed a significant group effect for cellular diameter, nuclear diameter and ratio of nuclear diameter to cellular diameter. Multiple comparison tests by Tukey-HSD procedure revealed a significant decrease in the mean cellular diameter, increase in the nuclear diameter and ratio of nuclear diameter to cellular diameter.
Cytomorphometric changes could be the earliest indicators of cellular alterations. There is progressive decrease in cellular diameter, increase in nuclear diameter and increase in ratio of nuclear diameter to cellular diameter in smears from all tobacco users, as compared to normal subjects. This indicates that there could be cause-effect relationship between tobacco and quantitative alterations.
本研究的目的是通过细胞形态计量学评估咀嚼烟草对颊黏膜的影响。
我们比较了正常受试者（N）的颊黏膜鳞状细胞的细胞直径（CD）、核直径（ND）以及核直径与细胞直径之比（N/C），与无病变的烟草使用者（A）、有烟草 - 石灰病变者（B）、白斑患者（C）和口腔鳞状细胞癌患者（D）的颊黏膜鳞状细胞的相应指标。研究组由125名年龄在21岁至75岁之间的患者组成，分为五组（N、A、B、C和D）。
N组、A组、B组、C组和D组的细胞直径（以微米为单位）平均值分别为72.86±5.26、68.30±3.02、62.13±3.29、57.75±4.66、54.51±4.66（p<0.01）。N组、A组、B组、C组和D组的核直径（以微米为单位）平均值分别为8.70±1.30、8.98±1.08、9.06±0.83、9.12±1.06和11.04±1.46（p<0.01）。N组、A组、B组、C组和D组的核直径与细胞直径之比的平均值分别为0.11±2.00、0.13±1.82、0.14±1.35、0.16±3.11、0.21±4.51（p<0.01）。单因素方差分析（ANOVA）显示细胞直径、核直径以及核直径与细胞直径之比存在显著的组间效应。通过Tukey - HSD程序进行的多重比较测试显示，细胞直径平均值显著降低，核直径以及核直径与细胞直径之比增加。
细胞形态计量学变化可能是细胞改变的最早指标。与正常受试者相比，所有烟草使用者涂片的细胞直径逐渐减小，核直径增加，核直径与细胞直径之比增加。这表明烟草与定量改变之间可能存在因果关系。
