分析和比较正常生育能力的供体和特发性弱精子症不育患者射出精子中电压依赖性阴离子通道 mRNA 的差异。
Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.
机构信息
Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
出版信息
J Assist Reprod Genet. 2010 Dec;27(12):719-24. doi: 10.1007/s10815-010-9466-8. Epub 2010 Sep 1.
PURPOSE
to analyze the abundance and difference of voltage-dependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.
METHODS
high motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation. Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility.
RESULTS
real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa. The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors. No significant differences of VDAC1 and VDAC3 mRNA levels were found between two groups.
CONCLUSION
the high abundance of VDAC2 mRNA seemed to have a positive correlation with low sperm motility. The abnormal expression of VDAC might be related to male infertility with idiopathic asthenozoospermia.
目的
分析正常生育能力的供体和特发性弱精子症的不育患者射出精液精子中电压依赖性阴离子通道（VDAC）mRNA 的丰度和差异。
方法
使用不连续 Percoll 梯度离心法分别从 36 名供体和 40 名患者的精液中分离高活力和低活力精子。通过实时 PCR 检测两组不同精子活力的三种 VDAC 亚型的 mRNA 丰度和差异。
结果
实时 PCR 表明成熟精子中存在三种 VDAC mRNA。患者射出精液精子中的 VDAC2 mRNA 水平明显高于供体。两组间 VDAC1 和 VDAC3 mRNA 水平无显著差异。
结论
VDAC2 mRNA 的高丰度似乎与精子活力低呈正相关。VDAC 的异常表达可能与特发性弱精子症的男性不育有关。
Zotero 插件