Graduate Institute of Biotechnology, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Diabetes Technol Ther. 2010 Oct;12(10):809-13. doi: 10.1089/dia.2010.0085.
Interleukin-10 (IL-10) polymorphic variants are linked with cytokine production and are involved in many chronic inflammatory diseases, including type 2 diabetes mellitus (T2DM). We investigated the hypothesis that IL-10 promoter polymorphisms may be associated with cytokine expressions involved in the progression of diabetic nephropathy (DN).
A total of 72 Taiwanese subjects were included in this study; along with a control group, patients had a diagnosis of DN lasting ≥2 years, and patients had a diagnosis of T2DM with normal renal functions lasting ≥5 years. Their IL-10 and tumor necrosis factor-α (TNF-α) genotyping and association of blood chemistry, plasma IL-10, TNF-α, and monocyte chemoattract protein-1 (MCP-1), and urinary MCP-1 were investigated.
The IL-10-(-592) genotype exhibited significant association with cytokine expressions in DN: significantly higher TNF-α and lower plasma IL-10 levels were observed in IL-10-(-592)AA, whereas a higher urine MCP-1 level was found in Taiwanese patients with the IL-10-(-592)CC genotype.
IL-10-(-592) promoter polymorphisms may influence IL-10 and MCP-1 production, which may be an indicator of nephropathy risk in Taiwanese T2DM patients.
白细胞介素-10（IL-10）多态性与细胞因子的产生有关，并参与许多慢性炎症性疾病，包括 2 型糖尿病（T2DM）。我们研究了这样一种假设，即 IL-10 启动子多态性可能与参与糖尿病肾病（DN）进展的细胞因子表达有关。
本研究共纳入 72 名台湾受试者；除对照组外，患者的 DN 诊断持续≥2 年，且患者的 T2DM 诊断持续≥5 年，肾功能正常。对他们的 IL-10 和肿瘤坏死因子-α（TNF-α）基因分型以及血液化学、血浆 IL-10、TNF-α 和单核细胞趋化蛋白-1（MCP-1）以及尿 MCP-1 的关联进行了研究。
IL-10-（-592）基因型与 DN 中的细胞因子表达有显著相关性：在 IL-10-（-592）AA 中观察到 TNF-α显著升高，血浆 IL-10 水平降低，而在 IL-10-（-592）CC 基因型的台湾患者中发现尿 MCP-1 水平升高。
IL-10-（-592）启动子多态性可能影响 IL-10 和 MCP-1 的产生，这可能是台湾 T2DM 患者肾病风险的一个指标。
