Kang Jessica, Liu Chia-Hsiung, Lee Chien-Nan, Li Hung-Yuan, Yang Chien-Wen, Huang Shu-Chien, Lin Shin-Yu, Jou Tzuu-Shuh
Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, Taiwan.
Department of Surgery, College of Medicine, National Taiwan University, Taipei, Taiwan.
Front Genet. 2019 Feb 18;10:89. doi: 10.3389/fgene.2019.00089. eCollection 2019.
The association of interleukin-10 (IL-10) polymorphism with diabetes and its complication was recently established, while there were few researches considering the potential role of IL-10 in gestational diabetes (GDM). This study aimed to investigate the association between IL-10 gene rs1800896 (-1082 A/G), rs1800871 (-819 T/C), rs1800872 (-592 A/C), and rs3021094 (3388 A/C) single nucleotide polymorphisms (SNPs) and GDM susceptibility. This study included 72 GDM patients and 100 healthy pregnant women. Direct sequencing of the products from polymerase chain reactions of the extracted genomic DNA from study subjects were conducted for analyzing IL-10 gene polymorphism and further genotype frequencies were compared. Plasma IL-10 concentration was measured by ELISA method. The results revealed no significant difference in -592 A/C, -819 T/C, and -1082 A/G genotypes. Significantly increased prevalence of A allele ( = 0.028, OR = 1.69, 95% CI = 1.081-2.64) and A/A genotype ( = 0.031, OR = 2.881, 95% CI = 1.145-7.250) at a previously un-characterized rs3021094 SNP were discovered in the GDM group. Increased IL-10 levels and insulin resistance were also related to the genotype of rs3021094. The risk of GDM was increased when IL-10 level was over 6.5 pg/ml. Our study demonstrated that A allele and A/A genotype of rs3021094 SNP in IL-10 gene were linked to increased risk for GDM, IL-10 plasma level and insulin resistance, which could be potential targets for early screening and detection of GDM.
白细胞介素-10（IL-10）基因多态性与糖尿病及其并发症的关联最近已被证实，然而，很少有研究考虑IL-10在妊娠期糖尿病（GDM）中的潜在作用。本研究旨在探讨IL-10基因rs1800896（-1082 A/G）、rs1800871（-819 T/C）、rs1800872（-592 A/C）和rs3021094（3388 A/C）单核苷酸多态性（SNP）与GDM易感性之间的关联。本研究纳入了72例GDM患者和100例健康孕妇。对研究对象提取的基因组DNA进行聚合酶链反应产物的直接测序，以分析IL-10基因多态性，并进一步比较基因型频率。采用酶联免疫吸附测定（ELISA）法测定血浆IL-10浓度。结果显示，-592 A/C、-819 T/C和-1082 A/G基因型无显著差异。在GDM组中，发现一个此前未被鉴定的rs3021094 SNP的A等位基因（P = 0.028，OR = 1.69，95%CI = 1.081 - 2.64）和A/A基因型（P = 0.031，OR = 2.881，95%CI = 1.145 - 7.250）的患病率显著增加。IL-10水平升高和胰岛素抵抗也与rs3021094的基因型有关。当IL-10水平超过6.5 pg/ml时，GDM风险增加。我们的研究表明，IL-10基因rs3021094 SNP的A等位基因和A/A基因型与GDM风险增加、IL-10血浆水平和胰岛素抵抗相关，这可能是GDM早期筛查和检测的潜在靶点。
