搅拌摩擦增材制造AZ31B镁合金-羟基磷灰石复合材料的生物矿化与生物相容性
biomineralization and biocompatibility of friction stir additively manufactured AZ31B magnesium alloy-hydroxyapatite composites.
作者信息
Ho Yee-Hsien, Man Kun, Joshi Sameehan S, Pantawane Mangesh V, Wu Tso-Chang, Yang Yong, Dahotre Narendra B
机构信息
Laboratory for Laser Aided Additive and Subtractive Manufacturing, Department of Materials Science and Engineering, University of North Texas, 1150 Union Circle 305310, Denton, TX, 76203-5017, USA.
Department of Biomedical Engineering, University of North Texas, 1150 Union Circle 305310, Denton, TX, 76203-5017, USA.
出版信息
Bioact Mater. 2020 Jun 30;5(4):891-901. doi: 10.1016/j.bioactmat.2020.06.009. eCollection 2020 Dec.
The present study aims to evaluate effect of hydroxyapatite (HA, Ca(PO)OH), a ceramic similar to natural bone, into AZ31B Mg alloy matrix on biomineralization and biocompatibility. The novel friction stir processing additive manufacturing route was employed to fabricate Mg-HA composites. Various HA contents (5, 10, 20 wt%) were incorporated into Mg matrix. Microstructural observation and chemical composition analysis revealed that refined Mg grains and dispersion of HA particles at micro/nanoscales were achieved in Mg-HA composites after the friction stir processing. The biomineralization evaluation were carried out using immersion experiments in simulated body fluid followed by mineral morphology observation and chemical composition analysis. The wettability measurements were conducted to correlate the biomineralization behavior. The results showed improvement in wettability and bone-like Ca/P ratio in apatite deposit on the composites compared to as-received Mg. In addition, the increase of blood compatibility, cell viability and spreading were found in the higher HA content composites, indicating the improved biocompatibility. Therefore, friction stir processed Mg-20 wt%HA composite exhibited the highest wettability and better cell adhesion among other composites due to the effect of increased HA content within Mg matrix.
本研究旨在评估类似于天然骨的陶瓷羟基磷灰石（HA，Ca(PO)OH）加入AZ31B镁合金基体对生物矿化和生物相容性的影响。采用新型搅拌摩擦加工增材制造路线制备镁-羟基磷灰石复合材料。将不同含量（5%、10%、20%重量）的羟基磷灰石加入镁基体中。微观结构观察和化学成分分析表明，搅拌摩擦加工后，镁-羟基磷灰石复合材料中实现了细化的镁晶粒以及微米/纳米尺度上羟基磷灰石颗粒的分散。生物矿化评估通过在模拟体液中进行浸泡实验，随后观察矿物质形态并分析化学成分来进行。进行润湿性测量以关联生物矿化行为。结果表明，与原始镁相比，复合材料上磷灰石沉积物的润湿性和类骨钙/磷比有所改善。此外，在较高羟基磷灰石含量的复合材料中发现血液相容性、细胞活力和细胞铺展增加，表明生物相容性得到改善。因此，由于镁基体中羟基磷灰石含量增加的影响，搅拌摩擦加工的镁-20%重量羟基磷灰石复合材料在其他复合材料中表现出最高的润湿性和更好的细胞粘附性。
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