Cordero Alexis B, Kwon Youngjoo, Hua Xiang, Godwin Andrew K
Department of Medical Oncology, Women's Cancer Program, Fox Chase Cancer Center, USA.
J Vis Exp. 2010 Aug 17(42):2125. doi: 10.3791/2125.
Human cancer and response to therapy is better represented in orthotopic animal models. This paper describes the development of an orthotopic mouse model of ovarian cancer, treatment of cancer via oral delivery of drugs, and monitoring of tumor cell behavior in response to drug treatment in real time using in vivo imaging system. In this orthotopic model, ovarian tumor cells expressing luciferase are applied topically by injecting them directly into the mouse bursa where each ovary is enclosed. Upon injection of D-luciferin, a substrate of firefly luciferase, luciferase-expressing cells generate bioluminescence signals. This signal is detected by the in vivo imaging system and allows for a non-invasive means of monitoring tumor growth, distribution, and regression in individual animals. Drug administration via oral gavage allows for a maximum dosing volume of 10 mL/kg body weight to be delivered directly to the stomach and closely resembles delivery of drugs in clinical treatments. Therefore, techniques described here, development of an orthotopic mouse model of ovarian cancer, oral delivery of drugs, and in vivo imaging, are useful for better understanding of human ovarian cancer and treatment and will improve targeting this disease.
原位动物模型能更好地体现人类癌症及对治疗的反应。本文描述了一种卵巢癌原位小鼠模型的建立、通过口服给药治疗癌症以及使用体内成像系统实时监测肿瘤细胞对药物治疗的反应。在这个原位模型中，通过将表达荧光素酶的卵巢肿瘤细胞直接注射到包裹每个卵巢的小鼠囊中来进行局部应用。注射萤火虫荧光素酶的底物D - 荧光素后，表达荧光素酶的细胞会产生生物发光信号。该信号由体内成像系统检测，从而实现对个体动物肿瘤生长、分布和消退的非侵入性监测。通过灌胃给药可将最大给药体积10 mL/kg体重的药物直接输送到胃中，这与临床治疗中的药物给药方式非常相似。因此，本文所述的技术，即卵巢癌原位小鼠模型的建立、药物口服给药和体内成像，有助于更好地理解人类卵巢癌及其治疗，并将改善对该疾病的靶向治疗。
