Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Piboonsongkram Road, Bangsue, Bangkok, 10800, Thailand.
J Ind Microbiol Biotechnol. 2011 Jan;38(1):93-9. doi: 10.1007/s10295-010-0834-2. Epub 2010 Sep 3.
Monascus purpureus is a biopigment-producing fungi whose pigments can be used in many biotechnological and food industries. The growth kinetics of biopigment production were investigated in a liquid fermentation medium in a 5-l stirred tank bioreactor at 30°C, pH 7, for 8 days with 100 rpm agitation and 1.38 × 10(5) N/m(2) aeration. Thai Monascus purpureus strains TISTR 3002, 3180, 3090 and 3385 were studied for color production, growth kinetics and productivity. Citrinin as a toxic metabolite was measured from the Monascus fermentation broth. The biopigment productions were detected from fermentation broth by scanning spectra of each strain produced. Results showed a mixture of yellow, orange and red pigments with absorption peaks of pigments occurring at different wavelengths for the four strains. It was found that for each pigment color, the color production from the strains increased in the order TISTR 3002, 3180, 3090, 3385 with 3385 production being approximately 10 times that of 3002. Similar results were found for growth kinetics and productivity. HPLC results showed that citrinin was not produced under the culture conditions of this study. The L*, a* and b* values of the CIELAB color system were also obtained for the yellow, orange and red pigments produced from the TISTR 3002, 3180, 3090 and 3385 strains. The colors of the pigments ranged from burnt umber to deep red.
红曲菌是一种生物色素产生真菌，其色素可用于许多生物技术和食品工业。在 30°C、pH7 的条件下，在 5L 搅拌罐生物反应器中，以 100rpm 的搅拌速度和 1.38×10(5)N/m(2)的通气量，在液体发酵培养基中研究了生物色素生产的生长动力学，发酵时间为 8 天。研究了泰国红曲菌菌株 TISTR3002、3180、3090 和 3385 的产色、生长动力学和生产力。从红曲菌发酵液中测量桔霉素作为一种毒性代谢产物。通过对各菌株发酵液的扫描光谱检测到生物色素的产生。结果表明，四种菌株产生的黄色、橙色和红色混合色素，其色素的吸收峰出现在不同的波长。结果发现，对于每种色素颜色，菌株的色素产量按 TISTR3002、3180、3090、3385 的顺序增加，其中 3385 的产量约为 3002 的 10 倍。生长动力学和生产力也得到了类似的结果。HPLC 结果表明，在本研究的培养条件下，桔霉素没有产生。还获得了 TISTR3002、3180、3090 和 3385 菌株产生的黄色、橙色和红色色素的 CIELAB 颜色系统的 L*、a和 b值。色素的颜色范围从焦棕到深红色。
