Venkatachalam Mekala, Shum-Chéong-Sing Alain, Dufossé Laurent, Fouillaud Mireille
Laboratoire de Chimie et Biotechnologie des Produits Naturels-EA 2212, Université de la Réunion, 15 Avenue René Cassin, CS 92003, F-97744 Saint-Denis Cedex 9, Ile de la Réunion, France.
Ecole Supérieure d'Ingénieurs Réunion Océan Indien-ESIROI, 2 Rue Joseph Wetzell, F-97490 Sainte-Clotilde, Ile de la Réunion, France.
Microorganisms. 2020 May 11;8(5):711. doi: 10.3390/microorganisms8050711.
30548 is a marine-derived pigment producing filamentous fungus, isolated from the La Réunion island, in the Indian Ocean. The objective of this study was to examine and optimize the submerged fermentation (SmF) process parameters such as initial pH (4-9), temperature (21-27 °C), agitation speed (100-200 rpm), and fermentation time (0-336 h), for maximum production of pigments (orange and red) and biomass, using the Box-Behnken Experimental Design and Response Surface Modeling (BBED and RSM). This methodology allowed consideration of multifactorial interactions between a set of parameters. Experiments were carried out based on the BBED using 250 mL shake flasks, with a 100 mL working volume of potato dextrose broth (PDB). From the experimental data, mathematical models were developed to predict the pigments and biomass yields. The individual and interactive effects of the process variables on the responses were also investigated (RSM). The optimal conditions for maximum production of pigments and biomass were derived by the numerical optimization method, as follows-initial pH of 6.4, temperature of 24 °C, agitation speed of 164 rpm, and fermentation time of 149 h, respectively.
30548是一种从印度洋留尼汪岛分离出的产色素海洋丝状真菌。本研究的目的是使用Box-Behnken实验设计和响应面建模（BBED和RSM）来研究和优化深层发酵（SmF）工艺参数，如初始pH值（4 - 9）、温度（21 - 27°C）、搅拌速度（100 - 200转/分钟）和发酵时间（0 - 336小时），以实现色素（橙色和红色）和生物量的最大产量。这种方法能够考虑一组参数之间的多因素相互作用。实验基于BBED，使用250 mL摇瓶，马铃薯葡萄糖肉汤（PDB）工作体积为100 mL。根据实验数据建立数学模型来预测色素和生物量产量。还研究了工艺变量对响应的个体和交互作用（RSM）。通过数值优化方法得出色素和生物量最大产量的最佳条件如下：初始pH值为6.4、温度为24°C、搅拌速度为164转/分钟、发酵时间分别为149小时。
