Yolken R H, Barbour B, Wyatt R G, Kalica A R, Kapikian A Z, Chanock R M
Science. 1978 Jul 21;201(4352):259-62. doi: 10.1126/science.208150.
Rotaviruses cause gastroenteritis in man and a wide variety of animal species. They cross-react in many immunologic tests and have a similar appearance by electron microscopy, making differentiation among them difficult. Rotaviruses derived from different host species were distinguished by postinfection serum blocking virus activity in an enzyme-linked immunosorbent assay (ELISA). Thirty-three rotavirus isolates from children living in three different parts of the world could not be differentiated by this technique, but they were distinct from four strains recovered from calves, and a series of strains isolated from piglets, foals, monkeys, and infant mice. The four bovine strains were similar, but they could be differentiated from the other animal strains, each of which exhibited a distinct pattern when tested by the ELISA blocking technique.
轮状病毒可引起人类及多种动物的胃肠炎。它们在许多免疫学检测中会发生交叉反应，并且通过电子显微镜观察有相似的外观，这使得区分它们变得困难。在酶联免疫吸附测定（ELISA）中，通过感染后血清阻断病毒活性来区分源自不同宿主物种的轮状病毒。来自世界三个不同地区儿童的33株轮状病毒分离株无法用该技术区分，但它们与从犊牛中分离出的4株毒株以及从仔猪、马驹、猴子和幼鼠中分离出的一系列毒株不同。这4株牛源毒株相似，但它们可与其他动物毒株区分开来，通过ELISA阻断技术检测时，每株动物毒株都呈现出独特的模式。
