School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, PR China.
Colloids Surf B Biointerfaces. 2010 Dec 1;81(2):629-33. doi: 10.1016/j.colsurfb.2010.08.015. Epub 2010 Aug 14.
In this work, a water-insoluble β-D-glucan (PCSG), isolated from Poria cocos, was carboxymethylated to create a water-soluble derivative named as CP. After free amino groups have been introduced, CP was covalently immobilized onto PU surface. The hydrophilicity and the concentration of carboxyl group on the modified PU surface were determined. The fibrinogen and albumin adsorption to the surface, in vitro blood compatibility, and antibacterial activity of the surface against Pseudomonas aeruginosa were evaluated. The water contact angle measurement indicated that the hydrophilicity of PU surface increased after modification. The fibrinogen adsorption of the modified PU surface decreased 51.5%, compared with control PU. CP immobilization could prolong the blood coagulation time was suggested by APTT experiment. Antibacterial activity experiments indicated that CP modified surface obviously suppressed the growth of P. aeruginosa. Thereby, CP immobilization improves blood compatibility of PU surface and introduces special antibacterial bioactivity.
在这项工作中，从多孔菌属中分离得到一种不溶于水的β-D-葡聚糖（PCSG），并对其进行羧甲基化处理，得到一种水溶性衍生物，命名为 CP。CP 被共价固定到 PU 表面上，引入了游离氨基后。对修饰后的 PU 表面的亲水性和羧基浓度进行了测定。评估了表面对纤维蛋白原和白蛋白的吸附、体外血液相容性以及对铜绿假单胞菌的抗菌活性。水接触角测量表明，修饰后 PU 表面的亲水性增加。与对照 PU 相比，改性 PU 表面的纤维蛋白原吸附减少了 51.5%。APTT 实验表明 CP 固定化可以延长凝血时间。抗菌活性实验表明 CP 修饰表面明显抑制了铜绿假单胞菌的生长。因此，CP 固定化提高了 PU 表面的血液相容性，并引入了特殊的抗菌生物活性。
